Strain Fitness in Escherichia coli BW25113 around b3067

Experiment: LB, Aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaG and rpoD are separated by 194 nucleotidesrpoD and ygjF are separated by 78 nucleotidesygjF and ileX are separated by 124 nucleotidesileX and yqjH are separated by 53 nucleotides b3066: dnaG - DNA primase (NCBI), at 3,209,129 to 3,210,874 dnaG b3067: rpoD - RNA polymerase sigma factor (NCBI), at 3,211,069 to 3,212,910 rpoD b3068: ygjF - G/U mismatch-specific DNA glycosylase (NCBI), at 3,212,989 to 3,213,495 ygjF b3069: ileX - tRNA-Ile (NCBI), at 3,213,620 to 3,213,695 ileX b3070: yqjH - predicted siderophore interacting protein (NCBI), at 3,213,749 to 3,214,513 yqjH Position (kb) 3211 3212 3213Strain fitness (log2 ratio) -3 -2 -1 0 1at 3210.912 kb on + strandat 3210.912 kb on + strandat 3210.997 kb on + strandat 3213.016 kb on + strandat 3213.058 kb on - strand, within ygjFat 3213.069 kb on - strand, within ygjFat 3213.276 kb on - strand, within ygjFat 3213.334 kb on + strand, within ygjFat 3213.334 kb on + strand, within ygjFat 3213.449 kb on - strandat 3213.497 kb on - strandat 3213.497 kb on - strandat 3213.692 kb on + strandat 3213.789 kb on + strandat 3213.868 kb on - strand, within yqjH

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, Aerobic
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3,210,912 + -0.7
3,210,912 + +1.4
3,210,997 + -0.0
3,213,016 + +0.2
3,213,058 - ygjF b3068 0.14 +0.4
3,213,069 - ygjF b3068 0.16 +0.2
3,213,276 - ygjF b3068 0.57 -0.9
3,213,334 + ygjF b3068 0.68 -3.3
3,213,334 + ygjF b3068 0.68 -0.1
3,213,449 - -0.5
3,213,497 - +0.2
3,213,497 - -0.5
3,213,692 + -0.1
3,213,789 + +0.5
3,213,868 - yqjH b3070 0.16 +0.1

Or see this region's nucleotide sequence