Experiment: LB, Aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt truD and ispF overlap by 4 nucleotides ispF and ispD overlap by 1 nucleotides ispD and ftsB are separated by 18 nucleotides
b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326
truD
b2746: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI), at 2,869,323 to 2,869,802
ispF
b2747: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (NCBI), at 2,869,802 to 2,870,512
ispD
b2748: ftsB - cell divison protein FtsB (NCBI), at 2,870,531 to 2,870,842
ftsB
Position (kb)
2869
2870 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2868.332 kb on - strand at 2868.421 kb on - strand, within truD at 2868.647 kb on + strand, within truD at 2868.647 kb on - strand, within truD at 2868.744 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.785 kb on - strand, within truD at 2868.785 kb on - strand, within truD at 2868.791 kb on + strand, within truD at 2868.845 kb on - strand, within truD at 2868.845 kb on - strand, within truD at 2868.930 kb on + strand, within truD at 2868.930 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.996 kb on - strand, within truD at 2869.037 kb on + strand, within truD at 2869.118 kb on - strand, within truD at 2869.118 kb on - strand, within truD at 2869.194 kb on + strand, within truD at 2869.194 kb on + strand, within truD at 2869.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Aerobic remove 2,868,332 - -0.5 2,868,421 - truD b2745 0.14 -0.3 2,868,647 + truD b2745 0.35 +0.9 2,868,647 - truD b2745 0.35 -0.0 2,868,744 + truD b2745 0.44 +0.4 2,868,779 + truD b2745 0.48 -0.0 2,868,779 + truD b2745 0.48 -0.4 2,868,785 - truD b2745 0.48 -0.5 2,868,785 - truD b2745 0.48 -0.1 2,868,791 + truD b2745 0.49 +0.2 2,868,845 - truD b2745 0.54 +0.1 2,868,845 - truD b2745 0.54 +0.9 2,868,930 + truD b2745 0.62 +0.4 2,868,930 + truD b2745 0.62 +1.6 2,868,986 + truD b2745 0.68 +0.2 2,868,986 + truD b2745 0.68 -1.4 2,868,996 - truD b2745 0.68 -0.5 2,869,037 + truD b2745 0.72 +3.8 2,869,118 - truD b2745 0.80 +0.3 2,869,118 - truD b2745 0.80 -1.5 2,869,194 + truD b2745 0.87 -1.0 2,869,194 + truD b2745 0.87 +0.3 2,869,292 - +0.9
Or see this region's nucleotide sequence