Strain Fitness in Escherichia coli BW25113 around b2746

Experiment: LB, Aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttruD and ispF overlap by 4 nucleotidesispF and ispD overlap by 1 nucleotidesispD and ftsB are separated by 18 nucleotides b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326 truD b2746: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI), at 2,869,323 to 2,869,802 ispF b2747: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (NCBI), at 2,869,802 to 2,870,512 ispD b2748: ftsB - cell divison protein FtsB (NCBI), at 2,870,531 to 2,870,842 ftsB Position (kb) 2869 2870Strain fitness (log2 ratio) -1 0 1 2 3 4at 2868.332 kb on - strandat 2868.421 kb on - strand, within truDat 2868.647 kb on + strand, within truDat 2868.647 kb on - strand, within truDat 2868.744 kb on + strand, within truDat 2868.779 kb on + strand, within truDat 2868.779 kb on + strand, within truDat 2868.785 kb on - strand, within truDat 2868.785 kb on - strand, within truDat 2868.791 kb on + strand, within truDat 2868.845 kb on - strand, within truDat 2868.845 kb on - strand, within truDat 2868.930 kb on + strand, within truDat 2868.930 kb on + strand, within truDat 2868.986 kb on + strand, within truDat 2868.986 kb on + strand, within truDat 2868.996 kb on - strand, within truDat 2869.037 kb on + strand, within truDat 2869.118 kb on - strand, within truDat 2869.118 kb on - strand, within truDat 2869.194 kb on + strand, within truDat 2869.194 kb on + strand, within truDat 2869.292 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, Aerobic
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2,868,332 - -0.5
2,868,421 - truD b2745 0.14 -0.3
2,868,647 + truD b2745 0.35 +0.9
2,868,647 - truD b2745 0.35 -0.0
2,868,744 + truD b2745 0.44 +0.4
2,868,779 + truD b2745 0.48 -0.0
2,868,779 + truD b2745 0.48 -0.4
2,868,785 - truD b2745 0.48 -0.5
2,868,785 - truD b2745 0.48 -0.1
2,868,791 + truD b2745 0.49 +0.2
2,868,845 - truD b2745 0.54 +0.1
2,868,845 - truD b2745 0.54 +0.9
2,868,930 + truD b2745 0.62 +0.4
2,868,930 + truD b2745 0.62 +1.6
2,868,986 + truD b2745 0.68 +0.2
2,868,986 + truD b2745 0.68 -1.4
2,868,996 - truD b2745 0.68 -0.5
2,869,037 + truD b2745 0.72 +3.8
2,869,118 - truD b2745 0.80 +0.3
2,869,118 - truD b2745 0.80 -1.5
2,869,194 + truD b2745 0.87 -1.0
2,869,194 + truD b2745 0.87 +0.3
2,869,292 - +0.9

Or see this region's nucleotide sequence