Experiment: LB, Aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt holA and rlpB overlap by 1 nucleotides rlpB and leuS are separated by 14 nucleotides leuS and ybeL are separated by 234 nucleotides ybeL and ybeQ are separated by 69 nucleotides
b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828
holA
b0641: rlpB - minor lipoprotein (NCBI), at 670,828 to 671,409
rlpB
b0642: leuS - leucyl-tRNA synthetase (NCBI), at 671,424 to 674,006
leuS
b0643: ybeL - hypothetical protein (NCBI), at 674,241 to 674,723
ybeL
b0644: ybeQ - orf, hypothetical protein (VIMSS), at 674,793 to 675,770
ybeQ
Position (kb) 671
672
673
674
675 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 670.785 kb on + strand at 670.837 kb on - strand at 670.880 kb on - strand at 670.915 kb on - strand, within rlpB at 670.922 kb on - strand, within rlpB at 671.394 kb on - strand at 671.404 kb on - strand at 671.404 kb on - strand at 674.089 kb on - strand at 674.089 kb on - strand at 674.128 kb on - strand at 674.258 kb on + strand at 674.258 kb on + strand at 674.320 kb on + strand, within ybeL at 674.320 kb on + strand, within ybeL at 674.351 kb on + strand, within ybeL at 674.395 kb on + strand, within ybeL at 674.434 kb on + strand, within ybeL at 674.434 kb on + strand, within ybeL at 674.509 kb on + strand, within ybeL at 674.569 kb on + strand, within ybeL at 674.569 kb on + strand, within ybeL at 674.875 kb on + strand at 674.891 kb on - strand, within ybeQ at 674.925 kb on - strand, within ybeQ at 674.925 kb on - strand, within ybeQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Aerobic remove 670,785 + +1.4 670,837 - +0.3 670,880 - +0.1 670,915 - rlpB b0641 0.15 -0.3 670,922 - rlpB b0641 0.16 -0.8 671,394 - -1.5 671,404 - -0.1 671,404 - -0.6 674,089 - +0.0 674,089 - -0.3 674,128 - +1.8 674,258 + -0.4 674,258 + +0.3 674,320 + ybeL b0643 0.16 +0.2 674,320 + ybeL b0643 0.16 +0.4 674,351 + ybeL b0643 0.23 +0.6 674,395 + ybeL b0643 0.32 -1.1 674,434 + ybeL b0643 0.40 -2.8 674,434 + ybeL b0643 0.40 -0.7 674,509 + ybeL b0643 0.55 -0.1 674,569 + ybeL b0643 0.68 -0.3 674,569 + ybeL b0643 0.68 +0.1 674,875 + +0.6 674,891 - ybeQ b0644 0.10 +0.1 674,925 - ybeQ b0644 0.13 -0.7 674,925 - ybeQ b0644 0.13 -0.8
Or see this region's nucleotide sequence