Experiment: LB, Aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrH and frr are separated by 291 nucleotides frr and dxr are separated by 91 nucleotides dxr and ispU are separated by 185 nucleotides ispU and cdsA are separated by 12 nucleotides
b0171: pyrH - uridylate kinase (NCBI), at 191,855 to 192,580
pyrH
b0172: frr - ribosome releasing factor (NCBI), at 192,872 to 193,429
frr
b0173: dxr - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (NCBI), at 193,521 to 194,717
dxr
b0174: ispU - undecaprenyl pyrophosphate synthase (NCBI), at 194,903 to 195,664
ispU
b0175: cdsA - CDP-diglyceride synthetase (VIMSS), at 195,677 to 196,534
cdsA
Position (kb) 193
194
195 Strain fitness (log2 ratio) -2
-1
0
1
2 at 192.628 kb on + strand at 192.628 kb on + strand at 192.700 kb on + strand at 192.720 kb on + strand at 192.728 kb on - strand at 192.728 kb on - strand at 192.730 kb on + strand at 192.736 kb on - strand at 192.802 kb on - strand at 192.802 kb on - strand at 192.805 kb on + strand at 192.815 kb on + strand at 192.815 kb on + strand at 192.815 kb on + strand at 192.823 kb on + strand at 192.823 kb on + strand at 192.838 kb on + strand at 192.842 kb on + strand at 193.455 kb on + strand at 194.726 kb on + strand at 194.748 kb on + strand at 194.767 kb on + strand at 194.767 kb on + strand at 194.767 kb on + strand at 194.767 kb on + strand at 194.803 kb on + strand at 194.803 kb on + strand at 194.872 kb on + strand at 194.872 kb on + strand at 194.872 kb on + strand at 195.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Aerobic remove 192,628 + +0.2 192,628 + -0.7 192,700 + +0.2 192,720 + -0.3 192,728 - +0.8 192,728 - +1.8 192,730 + -0.2 192,736 - +1.4 192,802 - +0.3 192,802 - +1.0 192,805 + +0.2 192,815 + -0.0 192,815 + +0.0 192,815 + -2.4 192,823 + -0.6 192,823 + +0.1 192,838 + -0.4 192,842 + -0.9 193,455 + +0.2 194,726 + -0.2 194,748 + -2.4 194,767 + -0.2 194,767 + -0.0 194,767 + -0.9 194,767 + -0.6 194,803 + +0.0 194,803 + -0.0 194,872 + -0.6 194,872 + +0.1 194,872 + -0.3 195,682 + -0.6
Or see this region's nucleotide sequence