Experiment: LB, Aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadS and btuF are separated by 37 nucleotides btuF and pfs overlap by 8 nucleotides pfs and dgt are separated by 83 nucleotides
b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624
yadS
b0158: btuF - vitamin B12-transporter protein BtuF (NCBI), at 177,662 to 178,462
btuF
b0159: pfs - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI), at 178,455 to 179,153
pfs
b0160: dgt - deoxyguanosinetriphosphate triphosphohydrolase (NCBI), at 179,237 to 180,754
dgt
Position (kb) 178
179
180 Strain fitness (log2 ratio) -2
-1
0
1
2 at 177.531 kb on + strand, within yadS at 177.578 kb on + strand at 177.596 kb on - strand at 177.596 kb on - strand at 177.616 kb on + strand at 177.616 kb on + strand at 177.625 kb on + strand at 177.625 kb on + strand at 177.697 kb on + strand at 177.714 kb on - strand at 177.726 kb on + strand at 177.735 kb on - strand at 178.094 kb on - strand, within btuF at 178.094 kb on - strand, within btuF at 178.138 kb on + strand, within btuF at 178.138 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.333 kb on + strand, within btuF at 178.440 kb on + strand at 178.440 kb on + strand at 178.448 kb on - strand at 178.452 kb on + strand at 179.187 kb on + strand at 179.228 kb on + strand at 179.291 kb on + strand at 179.378 kb on + strand at 179.467 kb on + strand, within dgt at 179.470 kb on - strand, within dgt at 179.470 kb on - strand, within dgt at 179.528 kb on + strand, within dgt at 179.528 kb on + strand, within dgt at 179.529 kb on + strand, within dgt at 179.551 kb on + strand, within dgt at 179.572 kb on + strand, within dgt at 179.572 kb on + strand, within dgt at 179.612 kb on + strand, within dgt at 179.680 kb on + strand, within dgt at 179.680 kb on + strand, within dgt at 179.755 kb on + strand, within dgt at 179.755 kb on + strand, within dgt at 179.791 kb on - strand, within dgt at 179.801 kb on - strand, within dgt at 179.802 kb on + strand, within dgt at 179.807 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on - strand, within dgt at 179.821 kb on - strand, within dgt at 179.821 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.882 kb on + strand, within dgt at 179.882 kb on + strand, within dgt at 179.882 kb on + strand, within dgt at 179.894 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.903 kb on + strand, within dgt at 179.907 kb on + strand, within dgt at 179.910 kb on - strand, within dgt at 179.974 kb on - strand, within dgt at 179.974 kb on - strand, within dgt at 179.976 kb on + strand, within dgt at 179.981 kb on + strand, within dgt at 180.011 kb on + strand, within dgt at 180.011 kb on + strand, within dgt at 180.022 kb on + strand, within dgt at 180.032 kb on - strand, within dgt at 180.044 kb on + strand, within dgt at 180.044 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.051 kb on + strand, within dgt at 180.051 kb on + strand, within dgt at 180.052 kb on - strand, within dgt at 180.054 kb on + strand, within dgt at 180.054 kb on + strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.059 kb on - strand, within dgt at 180.059 kb on - strand, within dgt at 180.062 kb on + strand, within dgt at 180.063 kb on + strand, within dgt at 180.064 kb on - strand, within dgt at 180.072 kb on - strand, within dgt at 180.072 kb on - strand, within dgt at 180.091 kb on + strand, within dgt at 180.106 kb on + strand, within dgt at 180.114 kb on - strand, within dgt at 180.116 kb on + strand, within dgt at 180.129 kb on + strand, within dgt at 180.134 kb on - strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.142 kb on - strand, within dgt
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Aerobic remove 177,531 + yadS b0157 0.85 +0.3 177,578 + -0.2 177,596 - -0.3 177,596 - -0.5 177,616 + -0.6 177,616 + -1.2 177,625 + -0.4 177,625 + -0.3 177,697 + +0.2 177,714 - +1.0 177,726 + +0.3 177,735 - -0.2 178,094 - btuF b0158 0.54 -0.1 178,094 - btuF b0158 0.54 +0.2 178,138 + btuF b0158 0.59 -0.3 178,138 - btuF b0158 0.59 -0.1 178,150 - btuF b0158 0.61 +0.5 178,150 - btuF b0158 0.61 +0.1 178,304 - btuF b0158 0.80 -0.8 178,304 - btuF b0158 0.80 -0.6 178,333 + btuF b0158 0.84 -1.5 178,440 + -0.3 178,440 + -0.6 178,448 - -0.3 178,452 + -0.1 179,187 + +0.2 179,228 + +0.3 179,291 + -0.7 179,378 + +0.1 179,467 + dgt b0160 0.15 +0.1 179,470 - dgt b0160 0.15 +0.4 179,470 - dgt b0160 0.15 +0.7 179,528 + dgt b0160 0.19 +0.1 179,528 + dgt b0160 0.19 +1.0 179,529 + dgt b0160 0.19 -0.3 179,551 + dgt b0160 0.21 -0.1 179,572 + dgt b0160 0.22 -0.7 179,572 + dgt b0160 0.22 +1.3 179,612 + dgt b0160 0.25 -1.9 179,680 + dgt b0160 0.29 +0.1 179,680 + dgt b0160 0.29 -0.5 179,755 + dgt b0160 0.34 -0.2 179,755 + dgt b0160 0.34 +0.0 179,791 - dgt b0160 0.36 -0.1 179,801 - dgt b0160 0.37 +1.8 179,802 + dgt b0160 0.37 -1.3 179,807 + dgt b0160 0.38 +0.1 179,813 + dgt b0160 0.38 +0.2 179,813 + dgt b0160 0.38 +0.2 179,813 + dgt b0160 0.38 -0.4 179,813 - dgt b0160 0.38 -0.0 179,821 - dgt b0160 0.38 -0.7 179,821 - dgt b0160 0.38 +0.8 179,847 - dgt b0160 0.40 +0.7 179,847 - dgt b0160 0.40 -0.5 179,847 - dgt b0160 0.40 -0.7 179,882 + dgt b0160 0.42 +0.3 179,882 + dgt b0160 0.42 -0.7 179,882 + dgt b0160 0.42 -0.6 179,894 + dgt b0160 0.43 +0.1 179,902 + dgt b0160 0.44 -1.5 179,902 + dgt b0160 0.44 +0.4 179,902 + dgt b0160 0.44 -0.4 179,902 + dgt b0160 0.44 -0.5 179,903 + dgt b0160 0.44 +0.1 179,907 + dgt b0160 0.44 -1.0 179,910 - dgt b0160 0.44 -0.5 179,974 - dgt b0160 0.49 -0.5 179,974 - dgt b0160 0.49 +1.8 179,976 + dgt b0160 0.49 -0.3 179,981 + dgt b0160 0.49 -0.2 180,011 + dgt b0160 0.51 +0.3 180,011 + dgt b0160 0.51 +0.4 180,022 + dgt b0160 0.52 -1.0 180,032 - dgt b0160 0.52 -0.7 180,044 + dgt b0160 0.53 +1.1 180,044 + dgt b0160 0.53 -0.2 180,046 + dgt b0160 0.53 -0.5 180,046 + dgt b0160 0.53 +0.5 180,046 + dgt b0160 0.53 -1.1 180,046 + dgt b0160 0.53 -0.6 180,046 + dgt b0160 0.53 -0.7 180,046 + dgt b0160 0.53 -0.7 180,046 + dgt b0160 0.53 -1.1 180,046 + dgt b0160 0.53 +0.1 180,051 + dgt b0160 0.54 +0.5 180,051 + dgt b0160 0.54 -0.7 180,052 - dgt b0160 0.54 +0.8 180,054 + dgt b0160 0.54 -1.2 180,054 + dgt b0160 0.54 -0.2 180,054 - dgt b0160 0.54 -0.5 180,054 - dgt b0160 0.54 -0.5 180,054 - dgt b0160 0.54 +0.5 180,054 - dgt b0160 0.54 +1.4 180,054 - dgt b0160 0.54 +0.1 180,054 - dgt b0160 0.54 +0.1 180,059 - dgt b0160 0.54 +0.0 180,059 - dgt b0160 0.54 +0.6 180,062 + dgt b0160 0.54 +0.7 180,063 + dgt b0160 0.54 +0.6 180,064 - dgt b0160 0.54 -0.1 180,072 - dgt b0160 0.55 +0.8 180,072 - dgt b0160 0.55 +0.8 180,091 + dgt b0160 0.56 -1.0 180,106 + dgt b0160 0.57 -0.6 180,114 - dgt b0160 0.58 +0.0 180,116 + dgt b0160 0.58 +0.4 180,129 + dgt b0160 0.59 -1.2 180,134 - dgt b0160 0.59 -1.5 180,139 + dgt b0160 0.59 +1.8 180,139 + dgt b0160 0.59 +0.1 180,139 + dgt b0160 0.59 -0.2 180,139 + dgt b0160 0.59 +1.4 180,139 + dgt b0160 0.59 +0.4 180,139 + dgt b0160 0.59 -0.5 180,139 + dgt b0160 0.59 +0.3 180,142 - dgt b0160 0.60 -1.5
Or see this region's nucleotide sequence