Specific Phenotypes for Escherichia coli BW25113 in nitrogen source experiments

Condition Gene Description Fitness Cons.
Adenosine b0133 pantoate--beta-alanine ligase (NCBI) -3.3  
Adenosine b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -3.3  
Adenosine b3459 hypothetical protein (NCBI) -3.1  
Adenosine b3643 ribonuclease PH (NCBI) +1.9 Yes
Adenosine b3869 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) -1.6  
Ammonium chloride b0133 pantoate--beta-alanine ligase (NCBI) -1.9 Yes
Ammonium chloride b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -2.5 Yes
Ammonium chloride b1682 cysteine desulfurase ATPase component (NCBI) +1.2  
Ammonium chloride b3459 hypothetical protein (NCBI) -1.7  
Cytidine b0133 pantoate--beta-alanine ligase (NCBI) -1.7  
Cytidine b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -2.1  
Cytidine b3292 zinc-responsive transcriptional regulator (NCBI) -2.0  
Cytidine b3459 hypothetical protein (NCBI) -1.7  
D-Alanine b0133 pantoate--beta-alanine ligase (NCBI) -2.5  
D-Alanine b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -2.0  
D-Alanine b0451 ammonium transporter (NCBI) -2.9 Yes
D-Alanine b0674 asparagine synthetase B (NCBI) -1.8  
D-Alanine b1189 D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) -3.3 Yes
D-Alanine b1190 alanine racemase (NCBI) -1.4 Yes
D-Alanine b3459 hypothetical protein (NCBI) -2.7  
D-Alanine b3869 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) -3.4  
D-Alanine b4054 tyrosine aminotransferase, tyrosine-repressible, PLP-dependent (NCBI) -1.1  
D-Serine b0131 aspartate 1-decarboxylase precursor (NCBI) -2.9  
D-Serine b0133 pantoate--beta-alanine ligase (NCBI) -2.6  
D-Serine b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -2.7  
D-Serine b1602 pyridine nucleotide transhydrogenase (NCBI) -1.5  
D-Serine b1702 phosphoenolpyruvate synthase (NCBI) -2.8  
D-Serine b2364 DNA-binding transcriptional dual regulator (NCBI) -3.8 Yes
D-Serine b2366 D-serine dehydratase (NCBI) -4.7 Yes
D-Serine b3292 zinc-responsive transcriptional regulator (NCBI) -1.6  
D-Serine b3454 ATP-binding component of leucine transport (VIMSS) +2.6  
D-Serine b3455 leucine/isoleucine/valine transporter subunit (NCBI) +2.4  
D-Serine b3456 leucine/isoleucine/valine transporter subunit (NCBI) +2.6 Yes
D-Serine b3457 leucine/isoleucine/valine transporter subunit (NCBI) +2.4 Yes
D-Serine b3458 leucine transporter subunit (NCBI) +1.6 Yes
D-Serine b3459 hypothetical protein (NCBI) -2.6  
D-Serine b3460 leucine/isoleucine/valine transporter subunit (NCBI) +2.1  
Gly-DL-Asp b0674 asparagine synthetase B (NCBI) -4.0  
Gly-DL-Asp b1338 putative aminohydrolase (EC 3.5.1.14) (VIMSS) +1.5  
Gly-DL-Asp b1988 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) -1.9 Yes
Gly-DL-Asp b3496 predicted transporter (NCBI) -1.5  
Gly-DL-Asp b3540 dipeptide transporter (NCBI) -1.1 Yes
Gly-DL-Asp b3541 dipeptide transporter (NCBI) -1.1 Yes
Gly-DL-Asp b3542 dipeptide transporter (NCBI) -1.4 Yes
Gly-DL-Asp b3543 dipeptide ABC transporter, membrane subunit (DppB) (from data) -1.4 Yes
Gly-DL-Asp b3544 dipeptide transporter (NCBI) -1.3 Yes
Gly-DL-Asp b4024 aspartate kinase III (NCBI) -1.1  
Gly-DL-Asp b4054 tyrosine aminotransferase, tyrosine-repressible, PLP-dependent (NCBI) -1.7  
Gly-Glu b0382 hypothetical protein (NCBI) +1.6  
Gly-Glu b0674 asparagine synthetase B (NCBI) -3.6  
Gly-Glu b1338 putative aminohydrolase (EC 3.5.1.14) (VIMSS) +2.0  
Gly-Glu b2916 chromosome replication initiation inhibitor protein (NCBI) +1.4  
Gly-Glu b3541 dipeptide transporter (NCBI) -1.4 Yes
Gly-Glu b3542 dipeptide transporter (NCBI) -1.2 Yes
Gly-Glu b3543 dipeptide ABC transporter, membrane subunit (DppB) (from data) -1.4 Yes
Gly-Glu b3544 dipeptide transporter (NCBI) -1.1 Yes
Glycine b0451 ammonium transporter (NCBI) -2.9 Yes
Glycine b0674 asparagine synthetase B (NCBI) -3.2  
Glycine b0932 aminopeptidase N (NCBI) -1.2  
Glycine b1661 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) -1.5  
Glycine b1814 L-serine deaminase I (NCBI) -3.7 Yes
L-Alanine b1190 alanine racemase (NCBI) -1.6 Yes
L-Alanine b2670 predicted inner membrane protein (NCBI) -1.7  
L-Alanine b3869 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) -1.3  
L-Arginine b0674 asparagine synthetase B (NCBI) -2.8  
L-Arginine b1188 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) +4.6  
L-Arginine b1745 succinylarginine dihydrolase (NCBI) -3.4 Yes
L-Arginine b1746 succinylglutamic semialdehyde dehydrogenase (NCBI) -3.2 Yes
L-Arginine b1747 arginine succinyltransferase (NCBI) -3.9 Yes
L-Arginine b1748 succinylornithine transaminase, PLP-dependent (NCBI) -4.1 Yes
L-Arginine b1782 scaffolding protein for murein synthesizing machinery (NCBI) +1.1  
L-Arginine b1783 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) +4.4  
L-Arginine b1784 yeaH component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) +4.2  
L-Arginine b1988 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) +2.0  
L-Arginine b2306 histidine/lysine/arginine/ornithine transporter subunit (NCBI) -1.1 Yes
L-Arginine b2307 histidine/lysine/arginine/ornithine transporter subunit (NCBI) -1.4 Yes
L-Arginine b2308 histidine/lysine/arginine/ornithine transporter subunit (NCBI) -1.4 Yes
L-Arginine b3869 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) -1.4  
L-Asparagine b1767 cytoplasmic asparaginase I (NCBI) -4.4 Yes
L-Aspartic Acid b0674 asparagine synthetase B (NCBI) -2.1  
L-Aspartic Acid b4138 C4-dicarboxylate antiporter (NCBI) -3.1 Yes
L-Glutamine b0133 pantoate--beta-alanine ligase (NCBI) -1.7  
L-Glutamine b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -1.5  
L-Glutamine b3459 hypothetical protein (NCBI) -1.7  
L-Serine b0112 aromatic amino acid transporter (NCBI) +1.1  
L-Serine b1814 L-serine deaminase I (NCBI) -5.1 Yes
L-Serine b3494 universal stress protein UspB (NCBI) -1.5  
Putrescine Dihydrochloride b0213 orf, hypothetical protein (VIMSS) -1.6  
Putrescine Dihydrochloride b0382 hypothetical protein (NCBI) +2.8  
Putrescine Dihydrochloride b0451 ammonium transporter (NCBI) -2.0 Yes
Putrescine Dihydrochloride b0563 DLP12 prophage; predicted protein (RefSeq) -1.1  
Putrescine Dihydrochloride b0674 asparagine synthetase B (NCBI) -5.5  
Putrescine Dihydrochloride b0848 predicted inner membrane protein (NCBI) -2.7  
Putrescine Dihydrochloride b1162 predicted DNA-binding transcriptional regulator (NCBI) -1.2  
Putrescine Dihydrochloride b1296 putative amino acid/amine transport protein (VIMSS) -4.7 Yes
Putrescine Dihydrochloride b1297 putative glutamine synthetase (EC 6.3.1.2) (VIMSS) -4.9 Yes
Putrescine Dihydrochloride b1298 probable amidotransferase subunit (VIMSS) -5.3 Yes
Putrescine Dihydrochloride b1299 DNA-binding transcriptional repressor (NCBI) -5.2  
Putrescine Dihydrochloride b1300 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) -5.3 Yes
Putrescine Dihydrochloride b1301 gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI) -6.9 Yes
Putrescine Dihydrochloride b1864 hypothetical protein (NCBI) -1.4  
Putrescine Dihydrochloride b1896 trehalose-6-phosphate synthase (NCBI) +1.5  
Putrescine Dihydrochloride b1983 hypothetical protein (NCBI) -1.6  
Putrescine Dihydrochloride b2181 hypothetical protein (NCBI) +4.6  
Putrescine Dihydrochloride b2326 hypothetical protein (NCBI) -1.6  
Putrescine Dihydrochloride b2517 predicted enzyme (NCBI) -2.4  
Putrescine Dihydrochloride b2812 hypothetical protein (NCBI) -1.1  
Putrescine Dihydrochloride b2916 chromosome replication initiation inhibitor protein (NCBI) -1.3  
Putrescine Dihydrochloride b3073 putrescine:2-oxoglutaric acid aminotransferase, PLP-dependent (RefSeq) -1.4 Yes
Putrescine Dihydrochloride b3075 DNA-binding transcriptional repressor (NCBI) +1.4  
Putrescine Dihydrochloride b3166 tRNA pseudouridine synthase B (NCBI) -2.8  
Putrescine Dihydrochloride b3252 conserved inner membrane protein (NCBI) -2.0  
Putrescine Dihydrochloride b3292 zinc-responsive transcriptional regulator (NCBI) -2.3  
Putrescine Dihydrochloride b3397 ADP-ribose diphosphatase (NCBI) +2.9  
Putrescine Dihydrochloride b3423 DNA-binding transcriptional repressor (NCBI) -3.0  
Putrescine Dihydrochloride b3432 glycogen branching enzyme (NCBI) -2.0  
Putrescine Dihydrochloride b3494 universal stress protein UspB (NCBI) +1.8  
Putrescine Dihydrochloride b3507 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) +1.0  
Putrescine Dihydrochloride b3527 predicted zinc-dependent peptidase (NCBI) -1.5  
Putrescine Dihydrochloride b3712 predicted phosphopantetheinyl transferase (RefSeq) -1.0  
Putrescine Dihydrochloride b3780 ATP-dependent RNA helicase (NCBI) -2.6  
Putrescine Dihydrochloride b3869 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) -3.3  
Putrescine Dihydrochloride b4022 23S rRNA pseudouridine synthase (NCBI) +1.0  
Putrescine Dihydrochloride b4024 aspartate kinase III (NCBI) +1.3  
Putrescine Dihydrochloride b4054 tyrosine aminotransferase, tyrosine-repressible, PLP-dependent (NCBI) -1.2  
Putrescine Dihydrochloride b4218 predicted inner membrane protein (NCBI) +4.1  
Putrescine Dihydrochloride b4235 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) +1.0  
Putrescine Dihydrochloride b4390 probable nadAB transcriptional regulator (VIMSS) -2.6  
Putrescine Dihydrochloride b4391 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) +1.3  
casamino acids b0133 pantoate--beta-alanine ligase (NCBI) -1.6  
casamino acids b0134 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) -2.0  
casamino acids b0425 2-dehydropantoate 2-reductase (NCBI) -1.3  
casamino acids b0774 adenosylmethionine--8-amino-7-oxononanoate transaminase (NCBI) -1.2  
casamino acids b1704 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) -1.3 Yes
casamino acids b3412 carboxylesterase of pimeloyl-CoA synthesis (NCBI) -1.1  
casamino acids b3459 hypothetical protein (NCBI) -1.8