Fitness data for PP_5356 in Pseudomonas putida KT2440

Protein VdlD
SEED: cytosolic long-chain acyl-CoA thioester hydrolase family protein

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB -0.6 -3.9 compare
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB -0.5 -3.4 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -2.0 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -2.3 compare
carbon source Heptanoic (C) -0.4 -2.6 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.4 -2.1 compare
temperature Growth at 25C -0.4 -1.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 -2.3 compare
carbon source ferulate (C); with MOPS -0.4 -1.5 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -1.9 compare
carbon source carbon source D-Glucose 55.5 mM -0.4 -1.5 compare
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +1.8 compare
temperature shift Temperature shift 30_to_34; with MOPS +0.4 +2.2 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM +0.4 +2.3 compare
temperature shift Temperature shift 10_to_25; with MOPS +0.4 +2.1 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.4 +2.4 compare
pH Growth at pH9 and (C) D-Glucose +0.4 +2.4 compare
pH Growth at pH8 and (C) D-Glucose +0.4 +1.7 compare
nitrogen source D-2-Aminobutyric (N) +0.4 +2.0 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM +0.4 +2.8 compare