Fitness data for PP_5143 in Pseudomonas putida KT2440

conserved membrane protein of unknown function
SEED: Protein of unknown function DUF81

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 1.3)


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group condition fitness t score  
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -3.4 -3.3 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -2.6 -3.5 compare
temperature Growth at 30C; with MOPS -2.3 -1.7 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.3 -2.5 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM -2.2 -3.5 compare
nitrogen source NAG (N); with MOPS -1.9 -1.4 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -1.9 -3.1 compare
carbon source ferulate (C); with MOPS -1.8 -1.5 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.6 -2.4 compare
phage P. putida South MOI 0.1 -1.5 -3.3 compare
phage P. putida Sci Big MOI 0.1 -1.5 -1.6 compare
phage P. putida South MOI 0.1 -1.5 -2.5 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 4-benzoyl-L-phenylalanine 5 mM -1.4 -1.9 compare
phage P. putida South MOI 10 -1.4 -2.1 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.4 -2.0 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -1.4 -2.3 compare
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.4 -1.8 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Phenylalanine 5 mM -1.3 -3.1 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.3 +1.2 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +1.6 +3.6 compare