Fitness data for PP_4945 in Pseudomonas putida KT2440

23S rRNA m(6)A2030 methyltransferase
SEED: Protein involved in catabolism of external DNA

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)

group	condition	fitness	t score
reactor	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay	-0.7	-2.7	compare
pH	pH 9	-0.7	-2.1	compare
pH	Growth at pH7 and (C) D-Glucose	-0.7	-1.4	compare
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant	-0.6	-2.8	compare
phage	P. put South MOI 10	-0.6	-1.9	compare
carbon source	L-Proline (C)	-0.6	-2.6	compare
pH	pH 5	-0.6	-2.0	compare
phage	P. putida Sci small MOI 0.1	-0.5	-1.6	compare
pH	Growth at pH6 and (C) D-Glucose	+0.6	+1.8	compare
carbon source	D-Glucose (C); with MOPS	+0.6	+2.0	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs	+0.6	+2.4	compare
nitrogen source	NAG (N); with MOPS	+0.6	+1.6	compare
carbon source	ferulate (C); with MOPS	+0.6	+2.0	compare
pH	Growth at pH6 and (C) Trisodium citrate	+0.6	+2.0	compare
pH	Growth at pH6 and (C) Trisodium citrate	+0.6	+2.3	compare
stress	Growth with Chloride 500 mM; with MOPS	+0.6	+2.8	compare
micoeukaryotes	C.elegans, mixed culture-1	+0.6	+2.7	compare
nitrogen source	2,6-diaminopimelic (N)	+0.8	+2.6	compare
nitrogen source	D-2-Aminobutyric (N)	+0.8	+2.3	compare
in planta	Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	+1.1	+2.3	compare