Fitness data for PP_4589 in Pseudomonas putida KT2440

D-isomer specific 2-hydroxyacid dehydrogenase family protein
SEED: D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.3)


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group condition fitness t score  
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.6 -4.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.5 -2.8 compare
phage P. putida Sci Big MOI 0.1 -0.5 -2.6 compare
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 -3.1 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -2.2 compare
nitrogen source Propiolactam (N) -0.4 -2.6 compare
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.4 -2.3 compare
stress Growth with Chloride 500 mM -0.4 -2.4 compare
carbon source DL-3-Hydroxybutyric (C) -0.3 -2.3 compare
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -2.4 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.3 -2.2 compare
phage P. putida Sci small MOI 0.1 -0.3 -1.7 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.3 -2.5 compare
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.3 -2.2 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.3 -2.1 compare
carbon source Crotonic (C) -0.3 -2.0 compare
nitrogen source Ammonium chloride (N) +0.3 +1.8 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay +0.3 +2.2 compare
carbon source D-Glucose (C) +0.4 +2.4 compare
pH Growth at pH6 and (C) D-Glucose +0.5 +2.9 compare