Fitness data for PP_4585 in Pseudomonas putida KT2440

RNA methyltransferase, TrmH family
SEED: tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.3)


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group condition fitness t score  
phage P. putida Sci small MOI 0.1 -0.7 -2.8 compare
pH Growth at pH9 and (C) Trisodium citrate -0.5 -2.3 compare
nitrogen source 2,6-diaminopimelic (N) -0.5 -2.3 compare
carbon source 3-methyl-1-butanol 0.25 vol% (C) -0.4 -1.7 compare
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -0.3 -2.2 compare
phage P. putida PUT 7 MOI 1 -0.3 -1.8 compare
carbon source 4-Aminobutyric 10 mM (C) -0.3 -1.8 compare
temperature shift Temperature shift 30_to_25; with MOPS -0.3 -1.6 compare
pH pH 5 +0.3 +1.7 compare
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +1.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 +1.8 compare
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +2.2 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.3 +2.2 compare
carbon source Tween 20 (C) +0.3 +1.9 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +2.3 compare
nitrogen source Propiolactam (N) +0.4 +2.3 compare
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +2.1 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +1.9 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +2.4 compare
nitrogen source D-2-Aminobutyric (N) +0.6 +2.5 compare