Fitness data for PP_4448 in Pseudomonas putida KT2440

conserved protein of unknown function
SEED: Bipolar DNA helicase HerA

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)

group	condition	fitness	t score
reactor	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.	-0.8	-3.5	compare
temperature shift	Temperature shift 10_to_30; with MOPS	-0.8	-3.2	compare
pH	Growth at pH6 and (C) Trisodium citrate	-0.6	-2.2	compare
temperature shift	Temperature shift 30_to_25; with MOPS	-0.5	-2.3	compare
stress	Growth with Chloride 500 mM; with MOPS	-0.5	-1.9	compare
pH	Growth at pH8 and (C) D-Glucose	-0.5	-2.8	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs	+0.5	+2.0	compare
in planta	Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	+0.5	+1.5	compare
carbon source	carbon source 3-O-methyl-glucose 50 mM	+0.5	+2.2	compare
carbon source	Myristic (C)	+0.5	+1.7	compare
supernatant	LB 0.1x with 55.5 mM D-Glucose with Supernatant: Acinetobacter	+0.5	+3.0	compare
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant	+0.6	+2.6	compare
phage	P. putida Sci small MOI 0.1	+0.6	+2.4	compare
nitrogen source	Propiolactam (N)	+0.7	+2.6	compare
phage	PUT8 MOI 10	+0.7	+3.5	compare
phage	P. putida Sci small MOI 0.1	+0.7	+3.2	compare
in planta	Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days	+0.8	+2.9	compare
in planta	Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days	+0.9	+2.7	compare
phage	P. putida PUT 7 MOI 1	+0.9	+4.3	compare
phage	P. putida Missley MOI 0.1	+1.0	+4.5	compare