Fitness data for PP_4070 in Pseudomonas putida KT2440

conserved protein of unknown function
SEED: DNA-directed RNA polymerase specialized sigma subunit, sigma24-like

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.5 -3.3 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.3 -3.7 compare
carbon source Myristic (C) -1.1 -3.3 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -1.1 -3.7 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.0 -3.2 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -1.0 -3.7 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.8 -2.3 compare
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.8 -2.4 compare
reactor_pregrowth Adaptation in M9 media timecourse 2; day 3 -0.7 -4.1 compare
carbon source 5-Hydroxydodecanoate (C) -0.7 -2.4 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.7 -1.6 compare
carbon source L-Valine (C) -0.7 -2.9 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -1.5 compare
pH Growth at pH7 and (C) D-Glucose -0.6 -2.1 compare
nitrogen source nitrate (N) -0.6 -1.5 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +3.0 compare
carbon source Crotonic (C) +0.7 +2.9 compare
carbon source Rhamnogalacturonan - from potato (C) +0.7 +2.9 compare
nitrogen source nitrate (N) +0.9 +2.0 compare
nitrogen source 2,6-diaminopimelic (N) +1.4 +3.2 compare