Fitness data for PP_4059 in Pseudomonas putida KT2440

fused trehalose synthase B/maltokinase
SEED: Trehalose synthase (EC 5.4.99.16)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)


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group condition fitness t score  
nitrogen source L-Leucine (N) -0.7 -5.0 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.7 -4.6 compare
reactor_pregrowth Adaptation in M9 media timecourse 3; day 3 -0.7 -5.1 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.7 -4.9 compare
nitrogen source Caprolactam (N) -0.6 -4.4 compare
reactor_pregrowth Adaptation in M9 media timecourse 2; day 3 -0.6 -4.7 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.6 -3.9 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.6 -3.5 compare
phage P. putida Sci small MOI 0.1 -0.6 -2.5 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -0.5 -3.9 compare
carbon source 1,3-Butandiol (C) +0.6 +5.5 compare
carbon source L-Leucine (C) +0.7 +4.7 compare
nitrogen source Propiolactam (N) +0.7 +5.2 compare
nitrogen source Propiolactam (N) +0.8 +5.6 compare
nitrogen source L-Phenylalanine (N) +0.8 +6.5 compare
nitrogen source L-Phenylalanine (N) +0.8 +7.1 compare
nitrogen source DL-2-Aminobutyric (N) +1.2 +8.9 compare
nitrogen source DL-2-Aminobutyric (N) +1.3 +9.0 compare
carbon source 1,3-Hexanediol (C) +2.4 +12.5 compare
carbon source 1,3-Hexanediol (C) +2.5 +13.2 compare