Fitness data for PP_3804 in Pseudomonas putida KT2440

conserved exported protein of unknown function
SEED: ABC transporter in pyoverdin gene cluster, periplasmic component

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.8)


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group condition fitness t score  
pH Growth at pH7 and (C) D-Glucose -2.2 -2.8 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.7 -4.0 compare
carbon source D-Glucose (C) -1.5 -2.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.3 -2.0 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 -3.7 compare
carbon source acetate (C) -1.1 -3.2 compare
temperature shift Temperature shift 30_to_21; with MOPS -1.1 -2.0 compare
pH pH 9 -1.1 -2.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 -3.6 compare
pH Growth at pH6 and (C) Trisodium citrate -1.0 -3.0 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -2.6 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.9 -2.6 compare
stress Growth with Chloride 200 mM; with MOPS -0.9 -2.4 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.9 -2.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.9 -1.5 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.8 -2.7 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.8 -3.2 compare
phage P. putida Missley MOI 0.1 -0.8 -1.5 compare
nitrogen source 2,6-diaminopimelic (N) -0.8 -2.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.8 +2.3 compare