Fitness data for PP_3549 in Pseudomonas putida KT2440

multidrug efflux transport system - membrane fusion protein
SEED: Membrane fusion component of tripartite multidrug resistance system
KEGG: multidrug resistance protein A

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
phage P. putida Sci small MOI 0.1 -0.8 -1.4 compare
phage P. putida Missley MOI 0.1 -0.8 -2.1 compare
nitrogen source Urea (N) -0.7 -2.7 compare
nitrogen source Urea (N); with MOPS -0.7 -1.9 compare
phage P. putida PUT 7 MOI 1 -0.7 -1.7 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 -1.9 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.6 -1.7 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -0.6 -2.5 compare
stress Growth with Chloride 200 mM -0.6 -1.8 compare
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.6 -2.4 compare
temperature shift Temperature shift 30_to_25; with MOPS -0.6 -2.7 compare
carbon source Glucose (C) (20mM) -0.6 -2.7 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +2.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.6 +2.0 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM +0.6 +2.5 compare
phage P. putida Sci Big MOI 0.1 +0.6 +1.6 compare
pH pH 7 +0.6 +1.7 compare
nitrogen source 2,6-diaminopimelic (N) +0.7 +1.9 compare
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.7 +2.4 compare
nitrogen source 2,6-diaminopimelic (N) +0.9 +2.0 compare