Fitness data for PP_3212 in Pseudomonas putida KT2440

Rieske 2Fe-2S family protein
SEED: Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
nitrogen source nitrate (N) -0.9 -2.3 compare
phage P. putida Sci small MOI 0.1 -0.8 -1.2 compare
temperature shift Temperature shift 30_to_34; with MOPS -0.8 -2.9 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.8 -1.7 compare
nitrogen source 2,6-diaminopimelic (N) -0.7 -1.4 compare
carbon source Trisodium citrate (C) -0.7 -1.7 compare
nitrogen source nitrogen source nitrite 1mM -0.6 -1.5 compare
nitrogen source Propandiamine (N) -0.6 -1.3 compare
carbon source D-Lysine 10 mM (C) -0.6 -2.6 compare
temperature shift Temperature shift 30_to_25; with MOPS -0.6 -1.9 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.6 -1.5 compare
pH Growth at pH9 and (C) Trisodium citrate -0.6 -1.4 compare
temperature shift Temperature shift 10_to_34; with MOPS -0.6 -2.2 compare
carbon source 4-Methyl-2-oxopentanoic (C) -0.6 -2.6 compare
stress MOPS minimal media_Glucose20mM with Chloride 200 mM -0.6 -1.6 compare
pH Growth at pH6 and (C) D-Glucose -0.6 -1.7 compare
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -1.6 compare
r2a control with 0.2% methanol R2A control with 0.2% methanol +0.6 +2.7 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +0.6 +2.4 compare
nitrogen source Propiolactam (N) +0.6 +2.4 compare