Fitness data for PP_3185 in Pseudomonas putida KT2440

Pet18 protein
SEED: Thiaminase II (EC 3.5.99.2)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.9)


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group condition fitness t score  
nitrogen source Propiolactam (N) -1.7 -2.4 compare
carbon source acetate (C) -1.5 -1.7 compare
pH Growth at pH6 and (C) D-Glucose -1.3 -1.3 compare
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.3 -1.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.2 -1.5 compare
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.2 -2.7 compare
nitrogen source nitrogen source nitrite 1mM -1.1 -1.4 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 -1.8 compare
nitrogen source NAG (N); with MOPS -1.0 -1.5 compare
phage P. putida Sci small MOI 0.1 -1.0 -1.7 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.0 -2.7 compare
temperature Growth at 30C; with MOPS -1.0 -1.5 compare
pH Growth at pH7 and (C) Trisodium citrate -1.0 -1.7 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.9 -2.6 compare
pH D-Glucose (C) pH 6.5 -0.9 -3.1 compare
nitrogen source 3,5-Dinitrosalicylic (N) -0.9 -3.4 compare
temperature shift Temperature shift 30_to_34; with MOPS -0.9 -1.8 compare
no stress control Growth with no stress -0.9 -1.1 compare
carbon source L-Phenylalanine 5 mM (C) -0.9 -2.6 compare
r2a control R2A control -0.9 -3.5 compare