Fitness data for PP_2994 in Pseudomonas putida KT2440

Oxidoreductase, FMN-binding
SEED: 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
phage P. putida Sci small MOI 0.1 -0.6 -1.7 compare
nitrogen source D-2-Aminobutyric (N) -0.6 -2.2 compare
nitrogen source nitrogen source nitrate 5mM -0.4 -1.9 compare
temperature shift Temperature shift 30_to_25 +0.4 +2.4 compare
phage P. put PUT 10 MOI 1 +0.4 +2.0 compare
carbon source 3-Hydroxyhexanoic (C) +0.5 +1.9 compare
temperature shift Temperature shift 10_to_30 +0.5 +2.3 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.5 +1.8 compare
pH LB 0.1x with 55.5 mM D-Glucose; pH 4.6 +0.5 +2.5 compare
pH Growth at pH8 and (C) D-Glucose +0.5 +2.7 compare
nitrogen source nitrogen source nitrate 5mM +0.5 +2.3 compare
temperature shift Temperature shift 10_to_30; with MOPS +0.5 +2.3 compare
temperature shift Temperature shift 30_to_21; with MOPS +0.5 +1.8 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +0.5 +2.8 compare
carbon source D-Glucuronic gamma lactone (C) +0.5 +2.3 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.5 +2.3 compare
nitrogen source Urea (N); with MOPS +0.5 +2.3 compare
phage P. putida Sci Big MOI 0.1 +0.6 +2.2 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.6 +2.7 compare
nitrogen source 2,6-diaminopimelic (N) +0.7 +2.3 compare