Fitness data for PP_1083 in Pseudomonas putida KT2440

(2Fe-2S)-binding protein
SEED: Bacterioferritin-associated ferredoxin
KEGG: bacterioferritin-associated ferredoxin

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.9)


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group condition fitness t score  
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -3.0 -7.3 compare
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.4 -6.6 compare
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -2.3 -4.3 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -2.0 -3.9 compare
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.9 -6.0 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.7 -4.0 compare
carbon source ferulate (C); with MOPS -1.7 -2.3 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -1.6 -3.8 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.6 -2.5 compare
pH Growth at pH6 and (C) D-Glucose -1.4 -2.1 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -1.3 -3.3 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -1.3 -3.7 compare
nitrogen source NAG (N); with MOPS -1.2 -1.3 compare
reactor_pregrowth Adaptation in M9 media timecourse 3; day 3 -1.2 -4.0 compare
temperature Growth at 30C; with MOPS -1.0 -1.3 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.0 -2.4 compare
nitrogen source no extra (N); with MOPS -1.0 -2.7 compare
temperature shift Temperature shift 30_to_25; with MOPS -1.0 -1.4 compare
reactor_pregrowth Adaptation in M9 media timecourse 1; day 3 -1.0 -3.9 compare
no phage control Time6-nophage -0.9 -2.9 compare