Fitness data for PP_0613 in Pseudomonas putida KT2440

Amidase family protein
SEED: Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)
KEGG: aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.4)


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group condition fitness t score  
temperature shift Temperature shift 10_to_30; with MOPS -0.6 -2.5 compare
pH Growth at pH9 and (C) Trisodium citrate -0.6 -2.0 compare
carbon source 4-Methyl-2-oxopentanoic (C) -0.6 -2.3 compare
pH Growth at pH7 and (C) Trisodium citrate -0.6 -1.9 compare
carbon source 5-Hydroxydodecanoate (C) -0.5 -3.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.5 -2.6 compare
nitrogen source no extra (N); with MOPS -0.5 -2.6 compare
carbon source ferulate (C); with MOPS -0.5 -2.0 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. -0.5 -2.0 compare
carbon source D-Glucose (C) -0.5 -1.7 compare
temperature shift Temperature shift 10_to_25; with MOPS -0.5 -2.0 compare
dilutelb LB 0.1x with 55.5 mM D-Glucose -0.4 -2.1 compare
temperature Growth at 30C; with MOPS -0.4 -1.9 compare
temperature shift Temperature shift 10_to_34; with MOPS -0.4 -1.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 -2.1 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 +1.8 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 +2.6 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.4 +2.0 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +2.5 compare
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +2.1 compare