Fitness data for PP_0562 in Pseudomonas putida KT2440

Methyl-accepting chemotaxis protein CtpL
SEED: Methyl-accepting chemotaxis protein I (serine chemoreceptor protein)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)


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group condition fitness t score  
nitrogen source D-2-Aminobutyric (N) -1.2 -3.3 compare
phage P. putida Sci Big MOI 0.1 -1.0 -2.6 compare
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.7 -2.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.7 -1.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 -2.0 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -2.3 compare
nitrogen source nitrogen source nitrate 5mM -0.5 -1.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.5 -1.6 compare
pH Growth at pH6 and (C) Trisodium citrate -0.5 -1.6 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.5 -1.6 compare
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.5 -1.7 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 +1.3 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Phenylalanine 5 mM +0.5 +3.0 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.5 +2.8 compare
carbon source D-Glucose (C) with 20AA_mix_minus_Tyr +0.5 +2.6 compare
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X +0.5 +2.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.6 +2.2 compare
temperature shift Temperature shift 30_to_25 +0.6 +2.3 compare
carbon source L-Lysine (C) +0.6 +2.7 compare
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM +0.8 +3.6 compare