Fitness data for b4235 in Escherichia coli BW25113

pmbA: predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI)
SEED: TldE protein, part of TldE/TldD proteolytic complex
KEGG: PmbA protein

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.8)


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group condition fitness t score  
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 -4.7 compare
TN_15CS_warm37C_5dbl_rep2 -1.2 -8.6 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.1 -4.1 compare
TN_15CS_warm37C_5dbl_rep1 -1.1 -8.5 compare
TN_15CS_15C_10dbl_rep2 -1.1 -8.4 compare
TN_15CS_15C_10dbl_rep3 -1.1 -7.9 compare
stress Spectinomycin 0.0125 mg/ml -1.0 -5.7 compare
carbon source D-Serine (C) -1.0 -6.5 compare
TN_15CS_15C_10dbl_rep1 -1.0 -7.5 compare
TN_15CS_warm37C_5dbl_rep3 -1.0 -8.1 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.0 -4.2 compare
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.9 -3.9 compare
stress Aluminum chloride 5 mM -0.9 -4.6 compare
TN_18CS_18C_10dbl_rep3 -0.9 -6.5 compare
TN_15CS_15C_5dbl_rep3 -0.9 -6.1 compare
TN_18CS_warm37C_5dbl_rep3 -0.8 -7.3 compare
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.8 -4.1 compare
TN_18CS_warm37C_5dbl_rep1 -0.8 -5.9 compare
stress Trimethoprim 0.0025 mM +0.9 +7.2 compare
nitrogen source Putrescine (N) +1.0 +8.3 compare