Fitness data for b0661 in Escherichia coli BW25113

miaB: isopentenyl-adenosine A37 tRNA methylthiolase (NCBI)
SEED: tRNA-i(6)A37 methylthiotransferase
KEGG: bifunctional enzyme involved in thiolation and methylation of tRNA

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.9)


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group condition fitness t score  
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.9 -8.8 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.9 -6.9 compare
phage T7_phage 0.00015625 MOI TC2 -1.7 -6.6 compare
phage T7_phage 0.0015625 MOI TC1 -1.6 -4.6 compare
phage T7_phage 0.00015625 MOI TC4 -1.5 -5.2 compare
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.4 -5.1 compare
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 -4.9 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 -4.4 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 -4.4 compare
motility outer cut, LB soft agar motility assay -1.1 -3.4 compare
nitrogen source Putrescine (N) -1.0 -6.5 compare
carbon source Acetate (C) +0.9 +5.9 compare
carbon source L-Malic (C) +0.9 +5.7 compare
control NoPredeControl +0.9 +6.8 compare
carbon source Ying_AminoAcid20 rep B; time point 3 +0.9 +7.5 compare
carbon source L-Fucose (C) +0.9 +6.9 compare
carbon source Ying_sugar7 rep C; time point 3 +1.0 +6.3 compare
carbon source Acetate (C) +1.1 +8.1 compare
carbon source Ying_sugar7 rep A; time point 3 +1.1 +9.4 compare
carbon source Ying_sugar7 rep B; time point 3 +1.1 +8.7 compare