Fitness data for b0331 in Escherichia coli BW25113

prpB: 2-methylisocitrate lyase (NCBI)
SEED: Methylisocitrate lyase (EC 4.1.3.30)
KEGG: methylisocitrate lyase

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.6)


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group condition fitness t score  
phage Ste41 -1.2 -2.4 compare
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.0 -2.1 compare
carbon source carbon source D-Glucose 0.25mM -1.0 -1.2 compare
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -1.0 -1.7 compare
carbon source Carbon source D-Glucose 10 mM -0.9 -5.3 compare
carbon source carbon source D-Glucose 1mM -0.7 -1.8 compare
stress octanol 0.08 vol% -0.7 -0.9 compare
carbon source L-Fucose (C) -0.7 -3.0 compare
stress Spectinomycin 0.00625 mg/ml -0.7 -2.5 compare
carbon source Ying_sugar7 rep A; time point 2 -0.7 -3.7 compare
carbon source D-Galacturonic Acid (C) MOPS -0.6 -2.3 compare
stress RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM -0.6 -3.3 compare
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.6 -1.6 compare
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.6 -1.8 compare
stress 2-nitrobenzenesulfonyl fluoride -0.6 -1.9 compare
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector +0.6 +1.4 compare
M9 Acetate 0.5% aerobic +0.6 +2.3 compare
phage N4_phage 0.009375 MOI TC2 +0.6 +3.0 compare
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.6 +1.7 compare
phage T7_phage 0.0015625 MOI TC1 +0.9 +2.0 compare