Pairwise Alignments
Query, 767 a.a., mammalian cell entry protein from Pseudomonas simiae WCS417
Subject, 877 a.a., MCE domain protein from Escherichia coli BL21
Score = 305 bits (780), Expect = 9e-87
Identities = 214/694 (30%), Positives = 349/694 (50%), Gaps = 32/694 (4%)
Query: 16 SAIWVLPLIALIIGGWLGWRAYSQQGIDIQVRFESGEGIQVNKTEVVYKGMPVGKVKALA 75
S W+LP IAL+I GWL W +Y +G + + F S +GI +T V Y+G+ VG V+ ++
Sbjct: 21 SPFWLLPFIALMIAGWLIWDSYQDRGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDIS 80
Query: 76 LDDEGNNRGVIATIEMNKDVDQYLKTNTRFWLVKPSVSLAGITGLETLVSGNYIAASPGD 135
L D+ R + + + D+ L+ T+FWLV P SLAG++GL+ LV GNYI PG
Sbjct: 81 LSDD--LRKIEVKVSIKSDMKDALREETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGK 138
Query: 136 GEPTRKFKALSEEPPLSDAKPGLHLTVKADRLGSLNRGSPVFYKQIQVGQVKSYLLSEDQ 195
G+ F AL +P L + ++A LGSLN GS V++++I VG+V Y ++ ++
Sbjct: 139 GKEQDHFVALDTQPKYRLDNGDLMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNK 198
Query: 196 STVEIKVYIEPTYASLVRKHTRFWNASGISIDANLSGVKVRSESLSSIVAGGIAFATPEN 255
V I V IE + LV+K +RFWN SG+ + ++SG KV+ ESL+++V G IAF +PE
Sbjct: 199 QGVVIDVLIERRFTDLVKKGSRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPE- 257
Query: 256 RKDSPPTDPSLPFRLYEDFDAAAAGIRVKVKLTDFEGLQAGRTPVMYKGIQVGSLKTLKV 315
+S P + F LYED + G+ +K++L GL A TP+MY+G++VG L L +
Sbjct: 258 --ESKPAEAEDTFGLYEDLAHSQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDL 315
Query: 316 DPDLSSANAELTLDPLAEDYLVQDTQFWVVKPSISLAGITGLEALVKGNYIAIRPGDKGT 375
+P E+T+DP L ++T+ + P +SL+ L AL+ G + PGD
Sbjct: 316 NPG-GKVTGEMTVDPSVVTLLRENTRIELRNPKLSLSD-ANLSALLTGKTFELVPGD--G 371
Query: 376 APQREFVARAKAPPLDLRSPGLHMVLFTDNLGSLDVGSPILYKQVKVGSVQSYQFSRKNK 435
P++EFV L L + L +D G P++ V+VG V + + +K
Sbjct: 372 EPRKEFVVVPGEKALLHEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLT--SK 429
Query: 436 QLVIGVHIEKEYENLVNGSTRFWNASGVTLTGGLTGGIQVKSESLASLMAGGIAFETPEP 495
+ V IE ++ LV G ++F S V + GL G++ S + + GGI P
Sbjct: 430 GVTFTVAIEPQHRELVKGDSKFVVNSRVDVKVGL-DGVEFLGASASEWINGGIRI-LPGD 487
Query: 496 NVPLKKRIPRFRLFADREAANQHG------TLVTIKVDRADGMRPGTPVRFKGLDVGKIE 549
+K P L+A+ E A ++ T V++ + ++ G+ V ++ +VG++
Sbjct: 488 KGEMKASYP---LYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVI 544
Query: 550 SVDLSADMQSVLLSARITQVADRIARAGSQFWV---VKPELG----LMKTSNLETLVTGQ 602
+V A+ + L I + + S FW K +L ++ S L + G
Sbjct: 545 TVRPRANAFDIDL--HIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGA 602
Query: 603 YIEVLPAAKNAGPQKSFVALDQPPEAVHQEAGLSLTLSAARRGSLKEGVPVTYREVTVGK 662
+ +A +K + E + G +TL A G L G+P+ Y + +G+
Sbjct: 603 ISFDNLSGASASQRKGDKRILYASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQ 662
Query: 663 VTGYELGQTADRVLIHILIEPKYA-PLVRSGSRF 695
+ +L + V ++ P+Y R G+RF
Sbjct: 663 IQTLDLITARNEVQAKAVLYPEYVQTFARGGTRF 696
Score = 232 bits (592), Expect = 6e-65
Identities = 206/749 (27%), Positives = 353/749 (47%), Gaps = 59/749 (7%)
Query: 43 DIQVRFESGEGIQVNKTEVVY-KGMPVGKVKALALDDEGNNRGVIATIEMNKDVDQYLKT 101
D+ + ++ + +N +VY + +PVGKV A++ N +GV+ + + + +K
Sbjct: 160 DLMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINP--NKQGVVIDVLIERRFTDLVKK 217
Query: 102 NTRFWLVK---PSVSLAG----ITGLETLVSGNYIAASPGDGEPTRKFKALSEEPPLSDA 154
+RFW V +VS++G + L LV+G SP + +P L+ +
Sbjct: 218 GSRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAHS 277
Query: 155 KPGLHLTVKADRLGSLNRGS-PVFYKQIQVGQVKSYLLSEDQSTVEIKVYIEPTYASLVR 213
+ G+ + ++ L S P+ Y+ ++VGQ+ L+ V ++ ++P+ +L+R
Sbjct: 278 QRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPG-GKVTGEMTVDPSVVTLLR 336
Query: 214 KHTRFWNASGISIDANLSGVKVRSESLSSIVAGGIAFATP---ENRKDSPPTDPSLPFRL 270
++TR I+ + + +LS+++ G P E RK+ P L
Sbjct: 337 ENTR--------IELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVV-PGEKALL 387
Query: 271 YEDFDAAAAGIRVKVKLTDFEGLQAGRTPVMYKGIQVGSLKTLKVDPDLSSANAELT--L 328
+E + + + + G+ AG+ P++ G+QVG + +D L+S T +
Sbjct: 388 HEP-----DVLTLTLTAPESYGIDAGQ-PLILHGVQVGQV----IDRKLTSKGVTFTVAI 437
Query: 329 DPLAEDYLVQDTQFWVVKPSISLAGITGLEAL-------VKGNYIAIRPGDKGT--APQR 379
+P + + D++F V G+ G+E L + G I I PGDKG A
Sbjct: 438 EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGG-IRILPGDKGEMKASYP 496
Query: 380 EFVARAKAPPLDLRS-PGLHMVLFTDNLGSLDVGSPILYKQVKVGSVQSYQFSRKNKQLV 438
+ KA L P + L + L + GS +LY++ +VG V + + +
Sbjct: 497 LYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVR--PRANAFD 554
Query: 439 IGVHIEKEYENLVNGSTRFWNASG--VTLTGGLTGGIQVKSESLASLMAGGIAFETPEPN 496
I +HI+ EY NL+ ++ FW G V L G G+ V++ L+ + G I+F+
Sbjct: 555 IDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGS---GLTVQASPLSRALKGAISFDNLSGA 611
Query: 497 VPLKKRIPRFRLFADREAANQHGTLVTIKVDRADGMRPGTPVRFKGLDVGKIESVDLSAD 556
+++ + L+A AA G +T+ A + G P+R+ G+D+G+I+++DL
Sbjct: 612 SASQRKGDKRILYASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITA 671
Query: 557 MQSVLLSARI-TQVADRIARAGSQFWVVKPELGLMKTSNLETLVTGQYIEVLPAAKNAGP 615
V A + + AR G++F VV P++ +L+T++ YI V P N P
Sbjct: 672 RNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQ-PYINVEPGRGN--P 728
Query: 616 QKSFVALDQPPEAVHQEAGLSLTLSAARRGSLKEGVPVTYREVTVGKVTGYELGQTADRV 675
++ F + GLS+ + A GSL G PV +R + VG VTG LG +DRV
Sbjct: 729 RRDFELQEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRV 788
Query: 676 LIHILIEPKYAPLVRSGSRFWNTSGFGLDFGLFKGATVRTESLETLVAGGIAFATPDGER 735
+I + I +Y LVR+ S FW SG+ LDFGL G V+T + + GGIAFATP G
Sbjct: 789 MIAMRISKRYQHLVRNNSVFWLASGYSLDFGL-TGGVVKTGTFNQFIRGGIAFATPPGTP 847
Query: 736 MGNPARPQQTFALFDKFEDEWLTWAPKIP 764
+ A+ + F L + EW W +P
Sbjct: 848 LAPKAQEGKHFLLQESEPKEWREWGTALP 876
Score = 149 bits (377), Expect = 5e-40
Identities = 89/239 (37%), Positives = 129/239 (53%), Gaps = 7/239 (2%)
Query: 513 EAANQHGTLVTIKVDRADGMRPG-TPVRFKGLDVGKIESVDLSADMQSVLLSARITQVAD 571
++ G VTI ADG+ PG TPVR++G++VG ++ + LS D++ + + I
Sbjct: 40 DSYQDRGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMK 99
Query: 572 RIARAGSQFWVVKPELGLMKTSNLETLVTGQYIEVLPAAKNAGPQKSFVALDQPPEAVHQ 631
R +QFW+V P+ L S L+ LV G YI ++P Q FVALD P+
Sbjct: 100 DALREETQFWLVTPKASLAGVSGLDALVGGNYIGMMPG--KGKEQDHFVALDTQPKYRLD 157
Query: 632 EAGLSLTLSAARRGSLKEGVPVTYREVTVGKVTGYELGQTADRVLIHILIEPKYAPLVRS 691
L + L A GSL G V +R++ VGKV Y + V+I +LIE ++ LV+
Sbjct: 158 NGDLMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKK 217
Query: 692 GSRFWNTSGFGLDFGLFKGATVRTESLETLVAGGIAFATPDGERMGNPARPQQTFALFD 750
GSRFWN SG + + GA V+ ESL LV G IAF +P+ + PA + TF L++
Sbjct: 218 GSRFWNVSGVDANVSI-SGAKVKLESLAALVNGAIAFDSPEESK---PAEAEDTFGLYE 272