Pairwise Alignments
Query, 1166 a.a., type VI secretion protein IcmF from Pseudomonas simiae WCS417
Subject, 1267 a.a., conserved membrane protein of unknown function from Pseudomonas putida KT2440
Score = 209 bits (531), Expect = 1e-57
Identities = 169/544 (31%), Positives = 261/544 (47%), Gaps = 46/544 (8%)
Query: 12 VIPVLGLIALSLIIWFVGPLLDVL---VPEGRRWALIILVFAVWIAYRVFRIIQARRQAA 68
++P+L L +L LI W PL+ L VPE A+I F + I R ++ I+A
Sbjct: 14 LLPLLWLASL-LICWLAAPLVPWLRHHVPEAL--AIISACFLLVIVLRQYQRIRAEHNLE 70
Query: 69 EVMRSLAAETPADPNSVATAEELTTLRQRMDEALALLKKAK-LGGDERRNLYELPWYVII 127
+++ E N+ + LR+R+ A+ +L+ + GG + L +LPWY+++
Sbjct: 71 NLLQ---IEVDRSWNATGEFRDQQVLRERLKHAITMLRTDRSAGGGGKAALSDLPWYLVV 127
Query: 128 GPPGSGKTTALVNSGLHFPLAAQLGAGAVRGVGGTRNCDWWFTDQAVLLDTAGRYTTQDS 187
G +GKT+ L +SGL +A A GT++CDW+F+ AV++DTAGRY D
Sbjct: 128 GMSAAGKTSLLTHSGLSASIAT-----ANDSESGTQHCDWYFSPDAVMIDTAGRYLRDDQ 182
Query: 188 NSTVDKAAWLGFLDLLKKQRSRRPIDGAFIAISLSDLLLGTDAERAAHAAAIRLRIQELY 247
+++ A FL +L+KQR + I+G + +SL +LL ER A AA + R++E
Sbjct: 183 SASEFSA----FLRMLRKQRGKAAINGLVLVVSLPELLAANSDERNALAAQLVARVEEYA 238
Query: 248 TQLGVRFPIYLMLTKLDLVPGFMEFFDNLSKEERAQVWGMTFALDDGKNTDSPLAHLQSE 307
L P+YLML+K D +PGF + FD L ER Q GMTF L + + T+ A LQ+
Sbjct: 239 ECLDANPPVYLMLSKTDQLPGFSQAFDGLDLHERQQPLGMTFGLSEIR-TNGLHAVLQTR 297
Query: 308 FAGLEQRLNERLVERLQQERDPARRDLIYGFPQQFGALKDCLQSFLEGVFKPNAFEERVL 367
L+ + + + ++ A L+ FPQ F AL L+ FLE + + +L
Sbjct: 298 LKKLQAHIRQHVDAQMIALGAEADSTLL-NFPQYFAALSGVLEQFLEHFTRGHRGGAPLL 356
Query: 368 LRGVYFTSGTQEGSPI-----DRLIGAMAQSMNLDRQ--HLARQSGTGRSYFIEKLFTAV 420
LRG+YFTS Q G + D + A D Q H+ + G RSYFI F V
Sbjct: 357 LRGLYFTSALQNGQQLGQVYEDVIADEFALQAAYDEQAGHVGKALG-NRSYFITDTFRQV 415
Query: 421 AFAERGLVGVNPKVERRRKW--IARGVLAATVALVVVVSSL-------WWVSYRANQAYI 471
F +R L+ ++ R+ + + G+ AAT L + +L W S R A+I
Sbjct: 416 IFPDRDLILYQSRLGRQAAFSPLLLGLAAATGLLFIGWQALSFANNRQWLDSLRGQLAHI 475
Query: 472 AQVDQKVAPLGQTVQNLSPAQRDILAVLPLLNAVKNLAGDSPSWSEGLGLYQGDMLEAES 531
Q + L A + + + + VK G GLYQG+ + +
Sbjct: 476 EQAADREQQLA--------AGKGLEVLREQMANVKAHRLQGVPLQLGGGLYQGEAIHQVT 527
Query: 532 ASVY 535
S Y
Sbjct: 528 RSAY 531
Score = 96.7 bits (239), Expect = 1e-23
Identities = 130/589 (22%), Positives = 227/589 (38%), Gaps = 53/589 (8%)
Query: 565 YEGLKAYLMLADSE-HYDPDFIKAWIALDWDRNLPRDLPADQRQALAG---HLQSLFERH 620
Y LK YL+L + + H DP F+ A + W PAD ++Q L +
Sbjct: 645 YNTLKLYLLLTEPQAHPDPAFVAASLPQAWASAAGEGTPADAGVIAENAPLYVQLLEQGQ 704
Query: 621 PPTARLDPRLIDDLRRQLQQLPVAQRVYDRVKRQKLPEG---IPDFRLNEAAGRDAALVF 677
P+ + +LI++ R+ L+ ++ + DR + E P LN+ + +
Sbjct: 705 APSLPRNEQLINETRQNLKSFMISSSLVDREYLRLQLESSRQFPALSLNDLVPQPGRALL 764
Query: 678 SRKSGKPLGEPLSGFFTAKGYRQAFLLSSLNQ--TGTLAEEQ-WVLGHEQADQQNVVSLA 734
+G P +T +G+ F+ L + +G L E WVL E D +V A
Sbjct: 765 YGTAGVP------AIYTRQGW-DTFVKPELIKLVSGNLRNESDWVLDGEGGDA--LVQKA 815
Query: 735 ADVRRL---YFQDYQRQWDALLADIDFVPITSVAQAADVLRVISG-PTSPLKKLLVAVAK 790
VR Y +DY + W +++ + +A A L ++S SP+K LL AV
Sbjct: 816 NFVREFMTRYKRDYTQAWYKMVSSVGVRHFADLASATRELGLLSDVQNSPVKNLLQAVND 875
Query: 791 ETDLQAEERQ-LAAKGVPVEGG----VDKLKERLGSLLGQEQPAANAPAAADDPVTAHFA 845
T +Q + A G + G V L + +L PA PA D + F
Sbjct: 876 NTQWDLPVKQAIPAPGTSRDDGFWSKVTGLFDANDTLPANVAPAL--PAVDDGSLAKRFE 933
Query: 846 ELNSIVSKNEGEPA---AIDGLLTDMNALYVQVSAM-----VGASGDALLGEAKNQAAAA 897
+ + ++N E A +D L + L V+++ + VG S L+ E +
Sbjct: 934 PVARVFAENNAEGADSTIMDRYLAALRKLKVRMNNIQRSQDVGKSSKQLISETLEGQPSE 993
Query: 898 ATRVSLNAERQPPL--------VQGMVKSVVNSTTNSMMGGVRNQLNAAWVSEVVNVYRQ 949
T V E +QG+ + ++ Q+ AW ++ + Q
Sbjct: 994 ITTVRNYVESSVDTSQDGLSRSLQGLFSLPIQYAWATLRDPAGEQIAKAWAQQIAKPWEQ 1053
Query: 950 SLAGRYPMSPGSARDATLDDFGQFFGV-GGVMDNYFRKYLQPYVDTSAQTWRWQPGAAQK 1008
+A RYP++ S +A++ D +F G++ + R + G A
Sbjct: 1054 VMAHRYPIAGSSRNEASVKDLQRFVDPDSGLLPAFKRNEIGNLAGGEGLGVGDGKGPAL- 1112
Query: 1009 LGIAPGVLQTFQRAATIRDAFFRAGGTQPIVRFELKPVSMDPTITQFLLDLDGQQLSYDH 1068
+ PG+L + +A+++ L+P T + LDGQ+ Y +
Sbjct: 1113 --VNPGMLNSIDKASSVGQVIASLSDRDNGFEIMLEP---SANFTDIVFTLDGQEQHYRN 1167
Query: 1069 GPSRPVAMQWPNPGSIGVVRISIMPPSASGRSGVTLDGPWAWFRLLEQS 1117
G S WP + R+ ++ S + + G W R+ E +
Sbjct: 1168 GRSSWNRFAWPGTSTAPGARLDVVTLSGTRVTVFDFPGRWGLLRMNESA 1216