Pairwise Alignments

Query, 554 a.a., NAD-dependent DNA ligase LigB from Pseudomonas simiae WCS417

Subject, 671 a.a., NAD-dependent DNA ligase LigA (NCBI) from Escherichia coli BW25113

 Score =  139 bits (349), Expect = 5e-37
 Identities = 143/587 (24%), Positives = 251/587 (42%), Gaps = 66/587 (11%)

Query: 24  DTARAQVNTLTEQIRLWDDSYHRLNQSPVSDELYDQAHQRLTHWRRCFPE--SPPKPDN- 80
           ++   Q+  L   +R  +  YH ++   + D  YD+  + L       PE  +P  P   
Sbjct: 2   ESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQR 61

Query: 81  ----PLASSRGTLRHPIAHTGLEKLLDEQAVGAWLSTRQD--------VWI-QPKVDGVA 127
               PLA+    +RH +    L+ + DE++  A+    QD         W  + K+DG+A
Sbjct: 62  VGAAPLAAF-SQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLA 120

Query: 128 VTLVYRQGRLRQVISRGDGLMGHDWSASARRIPGIVQQLPE---PIDLVLQGELYW---- 180
           V+++Y  G L    +RGDG  G D +++ R I  I  +L     P  L ++GE++     
Sbjct: 121 VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG 180

Query: 181 --HLDDHVQSANGGL--NARSKVAGLMNRLELSDADAAGIGLFVWA-----WPEGPGSFT 231
              +++  +   G +  N R+  AG + +L+        +  F +        E P +  
Sbjct: 181 FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL 240

Query: 232 ERLAALARWGFTDSRRYSQPIRDIAE---ATHWRAYWYNHPLPFASDGVVL--HQAQRPP 286
            RL    +WG   S R +  + + AE   A + +       L F  DGVV+  +   +  
Sbjct: 241 GRLLQFKKWGLPVSDRVT--LCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQE 298

Query: 287 ARRWKASEPYWAVAWKYPAAKALALVRNVRFKIGRTGRITPMLELEPVQLDDRQISRVSA 346
              + A  P WAVA+K+PA + +  VR+V F++GRTG ITP+  LEPV +    +S  + 
Sbjct: 299 QLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATL 358

Query: 347 GSLKRWQTLDIRPGDQVSISLAGQVIPRLDEVIL--RNAHRADLAAPDPRDFHALSCWQL 404
            +    + L +R GD+V I  AG VIP++  V+L  R     ++  P     H   C   
Sbjct: 359 HNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPT----HCPVCGSD 414

Query: 405 DPGCEEQLLARLT--------------WLSGNQGLALPHIGRETWNVLIQAGLIASFLDW 450
               E + +AR T                   + + +  +G +  + L++   + +  D 
Sbjct: 415 VERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADL 474

Query: 451 LTLDVAELANIEGFGDSSRTRVLESLNSARQRSFAQWLKALGVPPAARNNLE------GD 504
             L   +L  +E  G  S   V+ +L  A++ +FA++L ALG+               G 
Sbjct: 475 FKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGT 534

Query: 505 WQTLVARDTQAWLAIDGIGPGRAAQLSAFFRDPQVQALADTLRVAGI 551
            + L A   +    +  +G   A+ +  FF +   + +   L   G+
Sbjct: 535 LEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGV 581