Pairwise Alignments

Query, 1111 a.a., type VI secretion protein VasK from Pseudomonas simiae WCS417

Subject, 1267 a.a., conserved membrane protein of unknown function from Pseudomonas putida KT2440

 Score =  138 bits (348), Expect = 2e-36
 Identities = 147/576 (25%), Positives = 247/576 (42%), Gaps = 93/576 (16%)

Query: 11  VLRKSWVYSLLLVLSCALLVWFFGPLLAVDDYRFWQSPTSRLLTISGLLLLWGLAMVGMG 70
           +L+  W+   LL L+  L+ W   PL+          P  R      L ++    ++ + 
Sbjct: 7   LLKSFWLLLPLLWLASLLICWLAAPLV----------PWLRHHVPEALAIISACFLLVIV 56

Query: 71  ARRTARLNQPEEHERHQRRALINEEIRQ-------------VRARFKEALQALKTSRRYG 117
            R+  R+        H    L+  E+ +             +R R K A+  L+T R  G
Sbjct: 57  LRQYQRIRA-----EHNLENLLQIEVDRSWNATGEFRDQQVLRERLKHAITMLRTDRSAG 111

Query: 118 ERSERWRDELPWYLLIGEQGSGKTSLLAASGLPSPFDHPDAAPLGAASWCDWYFAEDAVL 177
              +    +LPWYL++G   +GKTSLL  SGL +     + +  G    CDWYF+ DAV+
Sbjct: 112 GGGKAALSDLPWYLVVGMSAAGKTSLLTHSGLSASIATANDSESGTQH-CDWYFSPDAVM 170

Query: 178 VEAAGRYLGQPDPSVDAAGWSTLLDLLKWRRRSRPLNGVVVTLSADTLSSSNEHDLERHA 237
           ++ AGRYL + D S  A+ +S  L +L+ +R    +NG+V+ +S   L ++N  +    A
Sbjct: 171 IDTAGRYL-RDDQS--ASEFSAFLRMLRKQRGKAAINGLVLVVSLPELLAANSDERNALA 227

Query: 238 RHVRTRLQDIQQTLHVDVPVYLVLTQSDRLAGFAEFFDAQQGDGTEQVLGGDLGTDLAQV 297
             +  R+++  + L  + PVYL+L+++D+L GF++ FD       +Q LG   G  L+++
Sbjct: 228 AQLVARVEEYAECLDANPPVYLMLSKTDQLPGFSQAFDGLDLHERQQPLGMTFG--LSEI 285

Query: 298 RD-----VFENLLQRLGGDLIPRLHQER---NVERRGHMLDFPRQVARIGDALCLFIEAA 349
           R      V +  L++L   +   +  +      E    +L+FP+  A +   L  F+E  
Sbjct: 286 RTNGLHAVLQTRLKKLQAHIRQHVDAQMIALGAEADSTLLNFPQYFAALSGVLEQFLEHF 345

Query: 350 FSVNRNQPINALRGFYLTCA---------------------------------KTVDVRP 376
              +R      LRG Y T A                                 K +  R 
Sbjct: 346 TRGHRGGAPLLLRGLYFTSALQNGQQLGQVYEDVIADEFALQAAYDEQAGHVGKALGNRS 405

Query: 377 HFVQGLFKRVIFAEADQAGLLTPERQRIHRRHGW--MALAATVVIGTVGAVW-AHSYSFN 433
           +F+   F++VIF + D    L   + R+ R+  +  + L      G +   W A S++ N
Sbjct: 406 YFITDTFRQVIFPDRD----LILYQSRLGRQAAFSPLLLGLAAATGLLFIGWQALSFANN 461

Query: 434 HQRLLQLTG-LIHTQTGTPPGADESHAL-----LTLLDSRLAATQVFSPQAQARWIDRAG 487
            Q L  L G L H +      AD    L     L +L  ++A  +    Q     +   G
Sbjct: 462 RQWLDSLRGQLAHIE----QAADREQQLAAGKGLEVLREQMANVKAHRLQGVPLQLG-GG 516

Query: 488 LYQGALSIAPLAAAYEHTLQQRFLPYVATVLEGRVR 523
           LYQG        +AY   L+ + L  +A  L+ ++R
Sbjct: 517 LYQGEAIHQVTRSAYLAQLRSQALEPIARSLQVQMR 552



 Score = 68.9 bits (167), Expect = 2e-15
 Identities = 138/650 (21%), Positives = 257/650 (39%), Gaps = 88/650 (13%)

Query: 485  RAGLYQGALSIAPLAAAYEHTLQQRFLPYVATVLEGRVRGSLGDREQLLDNLRAYLMLNL 544
            RAG Y   +++A  +   E +     L     +L       +    +  + L+ YL+L  
Sbjct: 597  RAGAYAAKVNLATASDGAELSAATGGLSLSEEMLGRLDEQQVASIIEAYNTLKLYLLLTE 656

Query: 545  RERR-DNAWLAEYVSGQWSG--------DSSANKRLNEHFARLLEQ---PFLAPLDEALV 592
             +   D A++A  +   W+         D+         + +LLEQ   P L P +E L+
Sbjct: 657  PQAHPDPAFVAASLPQAWASAAGEGTPADAGVIAENAPLYVQLLEQGQAPSL-PRNEQLI 715

Query: 593  ARARLVLRG----ESLAEVVYRALR-EQARNVEPYPLAE-----GPAFSRVEPPIPGFYT 642
               R  L+      SL +  Y  L+ E +R      L +     G A       +P  YT
Sbjct: 716  NETRQNLKSFMISSSLVDREYLRLQLESSRQFPALSLNDLVPQPGRALLYGTAGVPAIYT 775

Query: 643  RKYLQLFEQQGTQMVHAIA-----QDNWVL-GEGGDLGAADLRRLMLVLE--QRYFSEYA 694
            R+    F +   +++  ++     + +WVL GEGGD   A +++   V E   RY  +Y 
Sbjct: 776  RQGWDTFVKP--ELIKLVSGNLRNESDWVLDGEGGD---ALVQKANFVREFMTRYKRDYT 830

Query: 695  DVWSDALGRIRLRESGSLQQGAEYLASLTSAQSALVQ-LLQQVRENTRL----------- 742
              W   +  + +R    L      L  L+  Q++ V+ LLQ V +NT+            
Sbjct: 831  QAWYKMVSSVGVRHFADLASATRELGLLSDVQNSPVKNLLQAVNDNTQWDLPVKQAIPAP 890

Query: 743  -VSVSDRLETAVQPVAGLGAVTSRSLARAGLPDNARRALQRRFEPLHQLLDEQQNPGGEL 801
              S  D   + V  +         ++A A LP     +L +RFEP+ ++  E    G + 
Sbjct: 891  GTSRDDGFWSKVTGLFDANDTLPANVAPA-LPAVDDGSLAKRFEPVARVFAENNAEGADS 949

Query: 802  T---QALRLLDELQLQLSAVSRDSSPEQAAFKMARQRMDGQQPLLGNLR-------DTAA 851
            T   + L  L +L+++++ + R     +++ ++  + ++GQ   +  +R       DT+ 
Sbjct: 950  TIMDRYLAALRKLKVRMNNIQRSQDVGKSSKQLISETLEGQPSEITTVRNYVESSVDTSQ 1009

Query: 852  -RFPQPLKGWFEGIADQSWRQLLDDAYGFVNQHYQNEVYGFYAKAIQRRYPFDAHAGSDV 910
                + L+G F      +W  L D A   + + +  ++   + + +  RYP    + ++ 
Sbjct: 1010 DGLSRSLQGLFSLPIQYAWATLRDPAGEQIAKAWAQQIAKPWEQVMAHRYPIAGSSRNEA 1069

Query: 911  ALGDFQAFFKP-RGAMARFYESYLRPFVIVEGNRYRLRGIEGRSLPMSRSLLEQLTRAQI 969
            ++ D Q F  P  G +  F  + +    +  G    +   +G +L ++  +L  + +A  
Sbjct: 1070 SVKDLQRFVDPDSGLLPAFKRNEIGN--LAGGEGLGVGDGKGPAL-VNPGMLNSIDKASS 1126

Query: 970  IRQ---GFFTEDQG-----ELAVRFTLAPYSLDQAVSRATLRVGDQQLEYRHGPIVPVAF 1021
            + Q        D G     E +  FT   ++LD            Q+  YR+G      F
Sbjct: 1127 VGQVIASLSDRDNGFEIMLEPSANFTDIVFTLD-----------GQEQHYRNGRSSWNRF 1175

Query: 1022 HWP--NEAENGRSSLVLERGAERPLGIEKDSGAWSLFRFFDLMQSEPASG 1069
             WP  + A   R  +V   G    + +    G W L R  +  + +   G
Sbjct: 1176 AWPGTSTAPGARLDVVTLSGTR--VTVFDFPGRWGLLRMNESARVDDLDG 1223