Pairwise Alignments
Query, 863 a.a., General secretion pathway protein D from Variovorax sp. SCN45
Subject, 686 a.a., putative type II secretion protein GspD from Escherichia coli BL21
Score = 219 bits (557), Expect = 6e-61
Identities = 173/567 (30%), Positives = 278/567 (49%), Gaps = 76/567 (13%)
Query: 201 EVVKLRNGVAVDIAGLATELLEGSGEKGRRDIVVLADPRSNSVVI--RSSSPGRTTLARE 258
+VV +RN ++A + ++++ +G VV DP SN +++ R+S R T E
Sbjct: 146 KVVPVRNVSVRELAPILRQMIDSAGSGN----VVNYDP-SNVIMLTGRASVVERLT---E 197
Query: 259 LIAKLENAQSDPGNL--HVVYLRNAQSVHLAGVLRGLLTGESPDASGTGSGSVRAALGAG 316
+I ++++A GN V+ L NA + +A VL L + T + A
Sbjct: 198 VIQRVDHA----GNRTEEVIPLDNASASEIARVLESLTKNSGENQPATLKSQIVADERTN 253
Query: 317 GML-GGAGAANGTSGGAANASSANRGSTGGTGTGVSTMPSGG-----LRGVAGGASAGGT 370
++ G A + +G + L+ V+G +A
Sbjct: 254 SVIVSGDPATRDKMRRLIRRLDSEMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKE 313
Query: 371 GTQAGTSGNSNGAAFSAGGVTVQADATTNTLIIAAPEPMYRSLRRVIDTLDQRRAQVLVE 430
+ GT G+ V++ A +N LI+ AP+ + +SL+ VI+ LD RRAQV VE
Sbjct: 314 EAE-GTVGSGREV------VSIAASKHSNALIVTAPQDIMQSLQSVIEQLDIRRAQVHVE 366
Query: 431 SLIVEVTERDASELGIQWMAGGG------NGRGVRAGT------------------NFGG 466
+LIVEV E G+QW + NG + GT G
Sbjct: 367 ALIVEVAEGSNINFGVQWASKDAGLMQFANGTQIPIGTLGAAISQAKPQKGSTVISENGA 426
Query: 467 ATLNPNARNTIEAMPR------GLNIGIVDGTVNLPGVGEILNLKMLARALQAKDGANIL 520
T+NP+ + + + G +G+V G + L +A++ +N+L
Sbjct: 427 TTINPDTNGDLSTLAQLLSGFSGTAVGVVKG-----------DWMALVQAVKNDSSSNVL 475
Query: 521 STPNILTLDNEAASIMVGKTVPFVSGQYITNGNSSTNPFQTIQREDIGLKLNIRPQISEG 580
STP+I TLDN+ A MVG+ VP ++G T G++++NPF T++R+ +G+ L + PQI+EG
Sbjct: 476 STPSITTLDNQEAFFMVGQDVPVLTGS--TVGSNNSNPFNTVERKKVGIMLKVTPQINEG 533
Query: 581 GTVKLDIYQEVSTIDTQASDISGIVTNKRALDTSILVDDGQIMVLGGLMEDSVANGTEAV 640
V++ I QEVS ++ Q S +V +R L T++L +DG+++VLGGLM+D V
Sbjct: 534 NAVQMVIEQEVSKVEGQTS--LDVVFGERKLKTTVLANDGELIVLGGLMDDQAGESVAKV 591
Query: 641 PGLGALPVVGNLFRYDKRQRVKTNLMVFLRPYVIRDANAGRGLTLDRYNFMRAQQGRARP 700
P LG +P++GNLF+ ++ K NLMVF+RP ++RD A G++ +YN+MRA+Q
Sbjct: 592 PLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTILRDGMAADGVSQRKYNYMRAEQIYRDE 651
Query: 701 VPHGLLPDVGGPALPPAD--LPVSGRA 725
L+P P LP + LP RA
Sbjct: 652 QGLSLMPHTAQPVLPAQNQALPPEVRA 678
Score = 102 bits (253), Expect = 1e-25
Identities = 76/284 (26%), Positives = 138/284 (48%), Gaps = 14/284 (4%)
Query: 45 NFVDADITSVVSALARFLGRNFLFDPRVKGQITLVSEGEVPAATAYGMLSTALRMRGFAI 104
NF D D+ S + + L + + P V+G++++ + + Y + L +G+A+
Sbjct: 48 NFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEAQGYAV 107
Query: 105 VDV-GEVSRVVPVADAKLQG----GSVNTRNPGGGLATRTFRLNYENAEAMLPVLKPLIA 159
V + +V +VV + AK++ G + G + T+ + + + P+L+ +I
Sbjct: 108 VPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAPILRQMID 167
Query: 160 AENSINA--YPANNTLVVTDYIDNLERLARIIESIDTPTSLDAEVVKLRNGVAVDIAGLA 217
+ S N Y +N +++T +ERL +I+ +D + EV+ L N A +IA +
Sbjct: 168 SAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNASASEIARVL 227
Query: 218 TELLEGSGEKGRRDI--VVLADPRSNSVVIRSSSPGRTTLARELIAKLENAQSDPGNLHV 275
L + SGE + ++AD R+NSV++ S P R LI +L++ GN V
Sbjct: 228 ESLTKNSGENQPATLKSQIVADERTNSVIV-SGDPATRDKMRRLIRRLDSEMERSGNSQV 286
Query: 276 VYLRNAQSVHLAGVLR---GLLTGESPDASGT-GSGSVRAALGA 315
YL+ +++ L VL+ G LT +A GT GSG ++ A
Sbjct: 287 FYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAA 330
Score = 28.9 bits (63), Expect = 0.001
Identities = 32/134 (23%), Positives = 50/134 (37%), Gaps = 42/134 (31%)
Query: 137 TRTFRLNYENAEAMLPVLKP----LIAAEN-------------SINAYPANNTLVVTDYI 179
++ F L Y AE ++ VLK L AA+ SI A +N L+VT
Sbjct: 284 SQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHSNALIVTAPQ 343
Query: 180 DNLERLARIIESIDTP-------------------------TSLDAEVVKLRNGVAVDIA 214
D ++ L +IE +D S DA +++ NG + I
Sbjct: 344 DIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLMQFANGTQIPIG 403
Query: 215 GLATELLEGSGEKG 228
L + + +KG
Sbjct: 404 TLGAAISQAKPQKG 417