Pairwise Alignments

Query, 817 a.a., penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase from Pseudomonas putida KT2440

Subject, 803 a.a., Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) from Variovorax sp. SCN45

 Score =  511 bits (1315), Expect = e-149
 Identities = 305/834 (36%), Positives = 460/834 (55%), Gaps = 78/834 (9%)

Query: 4   LLKFFWWSS-------VAVICALVLGVSGAFLYLSPSLPSVESLRSIQLQIPLRVYSSDG 56
           LL+ F+W         ++V+C + + ++ A+    P+LP +  L   + ++PLRV+S++G
Sbjct: 26  LLRLFFWGFGIAAAGVLSVLCVVAVALAVAY----PNLPDISELSDYRPKLPLRVFSAEG 81

Query: 57  KLIAEFGEMRRSPIRFAEIPPQFIQALLSAEDDNFLNHYGVDPSSLMRAATQLVKTGHIQ 116
            LI EFGE RR+    + IP     A+L+AED  F +H GVD   ++RA   L     ++
Sbjct: 82  TLIGEFGEERRNLTPISAIPKVVKDAVLAAEDSRFYDHGGVDYKGMVRAG--LANLNRVK 139

Query: 117 T-GGSTITMQVAKNFFLTSERSFSRKTNEILLALQIERELTKDEILELYVNKIYLGNRAY 175
           + G STITMQVA+N +L+SE++ +RK  E+LL  ++E  LTKD+I E+Y+N+IYLGNRAY
Sbjct: 140 SQGASTITMQVARNVYLSSEKTLTRKVYEVLLTFKLEHLLTKDQIFEIYLNQIYLGNRAY 199

Query: 176 GIDAAAQVYYGKSIRDVSLAQMAMIAGLPKAPSRFNPLANPVRAKERRDWILGRMYKLGK 235
           G  AA++ Y+GK ++++++AQ AM+AGLPKAP   NP+ NP RA+ R+ +++ RM + G 
Sbjct: 200 GFAAASEAYFGKPLQELTIAQAAMLAGLPKAPGANNPVNNPQRARGRQFYVIDRMQEAGF 259

Query: 236 IDQASYEAALAEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSD 295
           I       A  E L+         ++A Y+AE  R  M  +YG   YT G +V T++ + 
Sbjct: 260 ITAEQAAEAKKEELHLRDAADPNRLHAEYVAETVRQLMYAQYGDSTYTRGLKVYTSLVAA 319

Query: 296 MQEMANKAVLKGLSDYDERHGYRGPEARFPGRTQAAWLQE-----LGKQRTLGGLEPAIV 350
            Q  A KA+ KG+ DY+ R  YRGPE        A  + E     L      G +  A+V
Sbjct: 320 DQAAAYKALRKGIMDYERRQIYRGPEKFVDLPNDAKDIDEAVDDALSDHPDNGDVMAAVV 379

Query: 351 TQVEHTGLKVLTRDGQEAQVAWDTMKWARPFINNNAQGRSPQSPADV-AQVGDLVRLQRL 409
            +     +  +  +G   Q+  + +K A+        G S ++P ++  + G ++R+ + 
Sbjct: 380 LKATPKQIDAVRANGDPVQITGEGLKPAQ-------SGLSDKAPPNIKIRRGAVIRVVKT 432

Query: 410 DDGTLKFSQVPVAQSALVTLDPNNGAIRALVGGFSFEQSNYNRAVQAKRQPGSSFKPFIY 469
              T + +Q+P  + A + +DP +GAI+ALVGGF F ++ +N   QA RQPGSSFKPFIY
Sbjct: 433 PKNTWEITQLPEVEGAFIAMDPRDGAIKALVGGFDFGKNKFNHVTQAWRQPGSSFKPFIY 492

Query: 470 SAALDSGYTASSLVNDAPIVF-VDESVDKVWRPKNDTNTFLGPIRMREALYKSRNLVSIR 528
           SAAL+ G+T ++++ND P+ F    +  + W PKN    + GP+ MR AL KS+NLVSIR
Sbjct: 493 SAALEKGFTPATVINDGPLFFDAGTTGGQPWEPKNYGGGYDGPMSMRTALMKSKNLVSIR 552

Query: 529 LLQAMGVDRTIDYIAKFGFNKQDLPRNLSLALGTATLTPMEIATGWSTFANGGYKITPYL 588
           +LQ++G     ++I  FGF+K   P  L +ALG  ++TPM++A G+S FANGGY++ PYL
Sbjct: 553 ILQSIGTRYAQEWITNFGFDKDKHPAYLPMALGAGSVTPMQMAVGYSVFANGGYRVNPYL 612

Query: 589 IERIESRSGETLFTANPARVPQGAQDQAGLAAPEQPISTAAMPGEAPSAFSQVAAASQAP 648
           + RI     + L    P                  P+   ++                  
Sbjct: 613 VTRITDHKDKVLVDKQP------------------PLLNESL------------------ 636

Query: 649 AVAEQIIDGRTTYILTSMLQDVIKRGTGRRALA-LGRTDLAGKTGTTNESKDAWFSGYNA 707
               + I  R  +I+ ++LQ V + GT  +A A L R DL GKTGTTN+S DAWF+G+  
Sbjct: 637 ----RAIPQRNAFIMDTLLQSVARAGTAAKAQAMLKRPDLYGKTGTTNDSLDAWFAGFQP 692

Query: 708 DYVTTVWVGFDQPETLGRREYGGTAALPIWMNFMGAALKDKPAH--APAEPEGILSLRVD 765
                 W+G+D P  LG RE GG  +LPIW+N+M  A+K  P    A A P GI++    
Sbjct: 693 TMTAISWIGYDTPRNLGDRETGGGLSLPIWINYMETAIKGVPVTDIATAPPAGIVN---- 748

Query: 766 PVSGR-AASPSTPNAYFELFKAEDSPPSVDELGNGAAPGSPLPADEATP-MDLF 817
            V G        P         E +P +  E   GA    P P +E +  +DLF
Sbjct: 749 -VGGEWYYDDYAPGRGVATLGVEAAPVAPVEALTGAPVSPPAPPEERSRILDLF 801