Pairwise Alignments

Query, 489 a.a., Type I restriction enzyme EcoEI M protein from Pseudomonas putida KT2440

Subject, 495 a.a., hypothetical protein from Xanthobacter sp. DMC5

 Score = 77.0 bits (188), Expect = 1e-18
 Identities = 127/503 (25%), Positives = 208/503 (41%), Gaps = 58/503 (11%)

Query: 12  DIMRKDVGVDGDAQRIGQLVWLLFLKIFDDRELEWELMDDNYKSPIPDSCRWR--TWAAD 69
           DI+R  +   GD +    +  LLF K   D    WE   + Y+  +    R+R  T AAD
Sbjct: 17  DILRGSID-SGDYKHY--IFGLLFFKRLSD---VWE---EEYEDRL---ARYRDATLAAD 64

Query: 70  PEGMTGDALKDFIDNNLFPQLQNLHEYSNTPSAFV------VRSVFEDAYNYMKSGQLLR 123
           PE    D  K     ++     ++  + N     +      +R VF+D  ++    +   
Sbjct: 65  PEEHRFDIPKGHFWKDIRRHTTDIGAHLNAAFHAIEDANQRLRGVFQDV-DFNNKERFPD 123

Query: 124 QVINKIQEGVDFNRAQ----ERHEFGNLYEQLL-RDLQNAGN-AGEFYTPRPVTEFMVRM 177
            ++ K+ +  + +R +    E    G  YE L+ R   ++G   GEFYTP+ V   +V  
Sbjct: 124 AMLEKLLQHFEAHRLRKADVEPDMLGQAYEYLIERFADDSGKKGGEFYTPKMVVRLIVEC 183

Query: 178 VDPKLAEKVMDPACGTGGFLTCAIEHKRRRYVKTAEDERTLQASIFGVEKKPLPHLLATT 237
           + P     + DP CG+GG L  A  H  R        +      +FG EK      +   
Sbjct: 184 LKPDEGMSIYDPTCGSGGMLLEAAHHLERL------GKNPKSIFLFGQEKNLNTWAICQM 237

Query: 238 NMILHGIEVPSQIRHDNTLSKPLISWGPSERV----HCIVANPPFG----GME---EDGI 286
           N+ LH I+  +++   +TL +P    G   +       ++ANPPF     G E   E   
Sbjct: 238 NLFLHDID-DAKVARGDTLLEPKHLTGEGVKAIRTFDRVLANPPFSLKSWGHEVWREGDA 296

Query: 287 ETNFPAAFRTRETADL-FLVLIMQLLKDGGRAAVVLPDGFLFGEGIKSRIKEKLLTECNL 345
                     +   DL F+  ++  LK  G   VVLP G LF  G + RI+E ++ +  +
Sbjct: 297 YGRDAYGCPPKSYGDLAFVQHMVASLKADGVCGVVLPHGVLFRGGAEGRIREGMIRDDLV 356

Query: 346 HTIVRLPNGVFNPYTGIKTNLLFFTKGTPTKQVWFYEHQYPAGVKNYSKTRPMRIEEFAV 405
             ++ L   +F    GI   +L   +  P  +    +     G + + + +       A 
Sbjct: 357 EAVIGLAPNLFYG-AGIPACILILRRKKPKDRRG--KVLIVNGAEQFVEGKAQNHLSEAN 413

Query: 406 EEAWWGSEADGFAARAENAFAWQVSVEELQARNWNLD-CKNPHTGEQV-SHDPDELLRNY 463
             A  G+ AD   A AE   A  V V E++A  +NL+  +  H GE+  + D  E +   
Sbjct: 414 VAALAGAYAD--FADAER-LARVVPVAEIEANAFNLNISRYVHLGEEAEAVDVAEEVEKL 470

Query: 464 ASVQDEISDLRDQLKAVLAEALR 486
            ++Q E    RD  +A +   LR
Sbjct: 471 VALQAE----RDAAEARMMGFLR 489