Pairwise Alignments

Query, 489 a.a., Type I restriction enzyme EcoEI M protein from Pseudomonas putida KT2440

Subject, 489 a.a., Type I restriction enzyme EcoEI methylase subunit from Escherichia coli ECOR38

 Score =  680 bits (1755), Expect = 0.0
 Identities = 331/489 (67%), Positives = 389/489 (79%), Gaps = 4/489 (0%)

Query: 1   MSISSTIKSIQDIMRKDVGVDGDAQRIGQLVWLLFLKIFDDRELEWELMDDNYKSPIPDS 60
           MSISS IKS+QDIMRKD GVDGDAQR+GQL WLLFLKIFD +E   EL  DNY+ PIP  
Sbjct: 1   MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQR 60

Query: 61  CRWRTWAADPEGMTGDALKDFIDNNLFPQLQNLHE-YSNTPSAFVVRSVFEDAYNYMKSG 119
             WR+WAA+ +G+TGD+L +F++++LFP L+NL       P  +VV+  F DAYNYMK+G
Sbjct: 61  YLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNG 120

Query: 120 QLLRQVINKIQEGVDFNRAQERHEFGNLYEQLLRDLQNAGNAGEFYTPRPVTEFMVRMVD 179
            LLRQVINK+ E +DF  A ERH FG++YEQ+L+DLQ+AGNAGEFYTPR VT FMV  VD
Sbjct: 121 TLLRQVINKLNE-IDFTSASERHLFGDIYEQILKDLQSAGNAGEFYTPRAVTRFMVDRVD 179

Query: 180 PKLAEKVMDPACGTGGFLTCAIEHKRRRYVKTAEDERTLQASIFGVEKKPLPHLLATTNM 239
           PKL E +MDPACGTGGFL CA +H + +YVK+  D +TLQ  I GVEKK LPHLLATTNM
Sbjct: 180 PKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKKQLPHLLATTNM 239

Query: 240 ILHGIEVPSQIRHDNTLSKPLISWGPSERVHCIVANPPFGGMEEDGIETNFPAAFRTRET 299
           +LHGIEVP QIRHDNTL+KPL SW   E++  IV NPPFGG EEDGIE NFPA  +TRET
Sbjct: 240 LLHGIEVPVQIRHDNTLNKPLSSW--DEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRET 297

Query: 300 ADLFLVLIMQLLKDGGRAAVVLPDGFLFGEGIKSRIKEKLLTECNLHTIVRLPNGVFNPY 359
           ADLFL LI+++L   GRAAVVLPDG LFGEG+K++IK+ L  ECNLHTIVRLPNGVFNPY
Sbjct: 298 ADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPY 357

Query: 360 TGIKTNLLFFTKGTPTKQVWFYEHQYPAGVKNYSKTRPMRIEEFAVEEAWWGSEADGFAA 419
           TGIKTNLLFFTKG PTK++WFYEH YPAGVKNYSKT+PM+ EEF  E  WWG+EADGFA+
Sbjct: 358 TGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFAS 417

Query: 420 RAENAFAWQVSVEELQARNWNLDCKNPHTGEQVSHDPDELLRNYASVQDEISDLRDQLKA 479
           R EN  AW+VS++++ ARN+NLD KNPH  E VSHDPDELL  YA  Q EI  LR+QL+ 
Sbjct: 418 RVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRD 477

Query: 480 VLAEALRRK 488
           +L  AL  K
Sbjct: 478 ILGAALSGK 486