Pairwise Alignments

Query, 787 a.a., Type I restriction enzyme EcoAI R protein from Pseudomonas putida KT2440

Subject, 818 a.a., Type I site-specific restriction-modification system, R (restriction) subunit and related helicases from Echinicola vietnamensis KMM 6221, DSM 17526

 Score =  938 bits (2424), Expect = 0.0
 Identities = 471/817 (57%), Positives = 601/817 (73%), Gaps = 36/817 (4%)

Query: 1   MDKKSLSERDICSKYIAPAIQHAGWDMHKQVREEVSFTKGRIMVRGKLHSRGEARRADFI 60
           +DKK+LSERDIC+K+I PAI+ AGWD   Q+ EEVSFT G+I VRG+L +RG+ +RAD+I
Sbjct: 2   IDKKTLSERDICTKFITPAIEKAGWDKTTQLLEEVSFTDGKIYVRGRLTARGKRKRADYI 61

Query: 61  LYHQANLPIAVIEAKDNKHSVGSGMQQALGYAEALDVPFVFSSNGDGFLFHDRSGTDSKV 120
           LY++ N+PIA++EAKDN+HSV +G+QQAL YAE LD+P VFSSNGDGF+FHDR+ TD  +
Sbjct: 62  LYYKPNIPIAIVEAKDNRHSVRAGLQQALDYAEILDIPCVFSSNGDGFVFHDRTATDGNI 121

Query: 121 ETELSLDQFPSPAELWHRYCQWKGLDNAVQHKIEAP-YYDDGSGRMPRYYQMNAINRTVE 179
           ETEL +D FPSP EL  +Y Q+KG+  +   ++    YY DGSGR PRYYQ  A+NRTVE
Sbjct: 122 ETELDIDSFPSPEELLKKYKQYKGIHTSEAQEVALQDYYFDGSGRSPRYYQQIAVNRTVE 181

Query: 180 AVARGQDRVLLVMATGTGKTYTAFQIIWRLWKSKQKKRILFLADRNILVDQTKNNDFKPF 239
           AVA GQ R+LLVMATGTGKTYTAFQII RLWKS+ KKRILFLADR  L+DQT+  DFK F
Sbjct: 182 AVANGQGRILLVMATGTGKTYTAFQIIHRLWKSRAKKRILFLADRTALIDQTRRGDFKHF 241

Query: 240 GQAMTKIARRQI--------------------DTSYEIYLSLYQAVTGAEEEMNVYKQFS 279
              MT I ++ +                    D +YE++L LYQ +T A +  + YK FS
Sbjct: 242 KDKMTIIKKKVVSRADGKEELVSNNKRGIDTEDKAYEVFLGLYQGLTNASDIEDAYKDFS 301

Query: 280 RDFFDLIVIDECHRGSAAEDSAWREILGYFSNATHVGLTATPKETKEVSSITYFGEPVYS 339
            +FFDLIVIDECHRGSA EDSAWREIL YF+ ATH+GLTATPKETKEVS+I YFG+P+Y+
Sbjct: 302 PEFFDLIVIDECHRGSAKEDSAWREILRYFNKATHIGLTATPKETKEVSNIEYFGDPIYT 361

Query: 340 YTLKQGIEDGFLAPYKVVRIDFDKDLQGWRPPKGMLDKNGELIEDRIYNLKDMDRTLVIE 399
           Y+LKQGI+DGFLAPY+VVR++ + D +GWRP +G  DK+G+ +EDR+YN KD D+TLVI+
Sbjct: 362 YSLKQGIDDGFLAPYRVVRVNLNVDAEGWRPEQGKKDKDGKEVEDRVYNRKDFDKTLVID 421

Query: 400 TRTQLVAQKVTEFLKASDPFQKTIVFCDDINHAERMRQALVNLNPERVAENRKYVMRITG 459
            RTQ VA+K+TEFLK  D F KTIVFC DI+HAERMR AL NLN + VA+N KY+M+ITG
Sbjct: 422 ERTQTVARKLTEFLKGYDRFAKTIVFCSDIDHAERMRFALSNLNADLVAKNHKYIMQITG 481

Query: 460 DDQEGKAELDNFINPEERYPVIATTSKLMTTGVDAQTCKLIVLDQHIKSMTEFKQIIGRG 519
           D+ EGK ELDNFINPEE YPVIATTS+LMTTGVDAQTC++IVLD  IKSMT+FKQI+GRG
Sbjct: 482 DNDEGKRELDNFINPEETYPVIATTSELMTTGVDAQTCRVIVLDAEIKSMTKFKQIVGRG 541

Query: 520 TRINEDFGKYWFTIMDFKKATELFADPAFDGDPV-VVYAPEGNELPVPPD---------- 568
           TRINE+FGK +FTI+DF+  T+LFAD  FDGDP+ V    E  +L    D          
Sbjct: 542 TRINEEFGKMFFTILDFRNVTDLFADKDFDGDPIRVKPVSEDTDLTNIVDEEQSIDSLII 601

Query: 569 DLLEGDGISAGSDGESDDLDFTGEEDGKKLIKYVIDSIPIYVIAERVQYYGPDGRLITES 628
           D   G+ I  G+     +      +  +   K  ++ + + V+  R  Y+  +G+ IT S
Sbjct: 602 DEETGEEIEIGTKIRYPEPQTRTSKVNEPRQKVYVNGVDVSVLISREMYFDTNGKPITTS 661

Query: 629 LHDYTRTCVQKQFASLDDFLRHWSDAEQKKVIIDEMAAQGVMWEALAEEVEKKQGKPLDP 688
           L D+T+  ++ Q+ASLDDFL  W+  ++K++II E+  QGV+ EAL + V ++    +D 
Sbjct: 662 LKDHTKELIKGQYASLDDFLARWNSTDKKEIIIKELEEQGVLVEALRDAVNRE----VDL 717

Query: 689 FDLICHVAFDQPPLSRKERAEQVKKRNYFAKYSGAARQVLEALLDKYADTGIEHIEDIKI 748
           FDLICHVAF+QPPL+RKERA  VKKR+YF KY   AR+VLE LLDKYAD G+ +IE + I
Sbjct: 718 FDLICHVAFEQPPLTRKERANNVKKRDYFTKYGDQARKVLETLLDKYADEGVTNIESMDI 777

Query: 749 LQLDPFNQLGAPIELVKAFGGKAGYNKAIHELEDALY 785
           L++ P    G+P+E++K FG KA Y +A+ ELE  LY
Sbjct: 778 LKVKPLTDYGSPLEIIKQFGSKAKYLEAVKELEQELY 814