Pairwise Alignments

Query, 565 a.a., long-chain-fatty-acid/CoA ligase from Pseudomonas putida KT2440

Subject, 561 a.a., acyl-CoA synthase (NCBI) from Escherichia coli BW25113

 Score =  625 bits (1611), Expect = 0.0
 Identities = 307/554 (55%), Positives = 407/554 (73%), Gaps = 3/554 (0%)

Query: 2   IENFWKDKYPAGITAEINPDEFPNIQAVLKQSCQRFADKPAFSNLGKTITYGELYALSGA 61
           ++  W ++YPA +  EINPD + ++  + +QS  R+AD+PAF N+G+ +T+ +L   S A
Sbjct: 1   MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVNMGEVMTFRKLEERSRA 60

Query: 62  FAAWLQQHTDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLIVVNTNPLYTAREMEHQFNDS 121
           FAA+LQQ   LK GDR+A+ +PN+LQYPVA+FG +RAG+IVVN NPLYT RE+EHQ NDS
Sbjct: 61  FAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDS 120

Query: 122 GAKALVCLANMAHLAEKVVPKTQVRHVIVTEVADLLPPLKRLLINSVIKYVKKMVPAYNL 181
           GA A+V ++N AH  EKVV KT V+HVI+T + D L   K  ++N V+KY+K++VP Y+L
Sbjct: 121 GASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHL 180

Query: 182 PQAVRFNDALALGKGQPVTEANPQANDVAVLQYTGGTTGVAKGAMLTHRNLVANMLQCRA 241
           P A+ F  AL  G      +      D+A LQYTGGTTGVAKGAMLTHRN++AN+ Q  A
Sbjct: 181 PDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNA 240

Query: 242 LMGSNLHEGCEILITPLPLYHIYAFTFHCMAMMLIGNHNVLISNPRDLPAMVKELGKWKF 301
             G  LH G E+++T LPLYHI+A T +C+  + +G  N+LI+NPRD+P +VKEL K+ F
Sbjct: 241 TYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPF 300

Query: 302 SGFVGLNTLFVALCNNEAFRALDFSALKITLSGGMALQLSVAERWKAVTGCAICEGYGMT 361
           +   G+NTLF AL NN+ F+ LDFS+L ++  GGM +Q  VAERW  +TG  + EGYG+T
Sbjct: 301 TAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLT 360

Query: 362 ETSPVAAVNPSEAN-QVGTIGIPVPSTLCKVIDDAGNELPLGEVGELCVKGPQVMKGYWQ 420
           E +P+ +VNP + +   G+IG+PVPST  K++DD  NE+P G+ GELCVKGPQVM GYWQ
Sbjct: 361 ECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVPPGQPGELCVKGPQVMLGYWQ 420

Query: 421 REDATAEILDSEGWLKTGDIAVIQADGYMRIVDRKKDMILVSGFNVYPNELEDVLAALPG 480
           R DAT EI+   GWL TGDIAV+  +G++RIVDRKKDMILVSGFNVYPNE+EDV+   PG
Sbjct: 421 RPDATDEII-KNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHPG 479

Query: 481 VLQCAAIGVPDEKSGEVIKVFIVVKPGMTVTKEQVMEHMRANVTGYKVPRHIEFRDSLPT 540
           V + AA+GVP   SGE +K+F VVK   ++T+E ++   R  +TGYKVP+ +EFRD LP 
Sbjct: 480 VQEVAAVGVPSGSSGEAVKIF-VVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPK 538

Query: 541 TNVGKILRRELRDE 554
           +NVGKILRRELRDE
Sbjct: 539 SNVGKILRRELRDE 552