Pairwise Alignments
Query, 680 a.a., Flagellar hook-associated protein FlgK from Pseudomonas putida KT2440
Subject, 547 a.a., flagellar hook-associated protein FlgK from Escherichia coli ECRC99
Score = 225 bits (573), Expect = 5e-63
Identities = 140/459 (30%), Positives = 243/459 (52%), Gaps = 16/459 (3%)
Query: 1 MSSLISIGLSGLSASQAALSVTSNNIANAATSGYSRQQTVQAAGPSHNIGAGFLGTGTTL 60
MSSLI+ +SGL+A+QAAL+ SNNI++ +GY+RQ T+ A S G++G G +
Sbjct: 1 MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYV 60
Query: 61 ADVRRIYSSYLDNQLQTATSLQADSVTFQDQITSVDKLLADRDTGISSVLTAFFSALQTA 120
+ V+R Y +++ NQL+ A + + +Q++ +D +L+ + +++ + FF++LQT
Sbjct: 61 SGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTL 120
Query: 121 AAKPGDVASRQLLLTQAQTLSNRFNAVSTQLTQQNATINSQLDTMAGQVNKLTANIAEYN 180
+ D A+RQ L+ +++ L N+F L Q+ +N + Q+N IA N
Sbjct: 121 VSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLN 180
Query: 181 KQITAAS--ASGNTPNSLLDARSEAVRQLNELVGVTVQERD-GNYDVYLGSGQSLVTGTK 237
QI+ + +G +PN+LLD R + V +LN++VGV V +D G Y++ + +G SLV G+
Sbjct: 181 DQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGST 240
Query: 238 ANTLSVEPGVADKSQVALRINYDSFSSDV---TSVVSGGAIGGLLRYRQDVLTPSMNELG 294
A L+ P AD S+ + D + ++ +++ G++GG+L +R L + N LG
Sbjct: 241 ARQLAAVPSSADPSRTTVAY-VDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLG 299
Query: 295 RVALVVADSINSQLGQGLDANGQFGSSLFSSINSATAIAQRSLASSNNSAGSGNLDVTIA 354
++AL A++ NSQ G DANG G F AI + ++ + + G+ + T+
Sbjct: 300 QLALAFAEAFNSQHKAGFDANGDEGEDFF-------AIGKPAVLQNTKNNGNVAIGATVT 352
Query: 355 NSGALTTYDYEVKFTSASQYSVRRSDGTDMGSFDLNADPAPVIDGFSLALKGGGLAAGDS 414
++ A+ DY++ F + +Q+ V R + +A+ DG L G A DS
Sbjct: 353 DASAVLATDYKISFDN-NQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTP-AVNDS 410
Query: 415 FKVIPTRAAAGSITTTLTDANKLAFAGPISAAAGSGNSG 453
F + P A ++ +TD K+A A A +G
Sbjct: 411 FTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNG 449
Score = 102 bits (253), Expect = 6e-26
Identities = 146/594 (24%), Positives = 239/594 (40%), Gaps = 93/594 (15%)
Query: 122 AKPGDVASRQLLLTQAQTLSNRFNAVSTQLTQQNATINSQLDT--MAGQVNKLTANIAEY 179
A G A++ L T + +S+ A T+ T A NS L G ++ EY
Sbjct: 8 AMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGVQREY 67
Query: 180 NKQITAASASGNTPNSLLDARSEAVRQLNELVGVTVQERDGNYDVYLGSGQSLVTGTK-- 237
+ IT + T +S L AR E + +++ ++ + + S Q+LV+ +
Sbjct: 68 DAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVSNAEDP 127
Query: 238 -ANTLSVEPGVADKSQVALRINYDSFSSDVTSVVSGGAIGGLLRYRQDVLTPSMNELGRV 296
A + +Q Y ++ G ++ + Y + + + + +++ R+
Sbjct: 128 AARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLN-DQISRL 186
Query: 297 ALVVADSINSQLGQGLDANGQFGSSLFSSINSATAIAQRSLASSNNSAGSGNLDVTIANS 356
V A + + L LD Q S L + ++ G ++T+AN
Sbjct: 187 TGVGAGASPNNL---LDQRDQLVSELNQIVGVEVSVQD-----------GGTYNITMANG 232
Query: 357 GAL----TTYDYEVKFTSA--SQYSVRRSDGTDMGSFDLNADPAPVIDGFSLALKGGGLA 410
+L T +SA S+ +V DGT G+ ++ P +++ SL GG+
Sbjct: 233 YSLVQGSTARQLAAVPSSADPSRTTVAYVDGT-AGNIEI---PEKLLNTGSL----GGIL 284
Query: 411 AGDSFKVIPTRAAAGSITTTLTDANKLAFAGPISAAAGSGNSGTGTITQPTLGESLDIYG 470
S + TR G + LAFA ++ +G G E D +
Sbjct: 285 TFRSQDLDQTRNTLGQLA--------LAFAEAFNSQHKAGFDANGD-------EGEDFFA 329
Query: 471 GADTALIQQSIRDSMPV--RVVFDAASGGSQGYKL-FDAKGTQIGTGSVVPGQDNKLSIA 527
A++Q + + V DA++ + YK+ FD Q+ + N
Sbjct: 330 IGKPAVLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLA-----SNTTFTV 384
Query: 528 VPMLDAAGDPILDGSGNPRTFAVETTIGGSPAANDSFTLSFNAD---------------- 571
P DA G DG +E T G+PA NDSFTL +D
Sbjct: 385 TP--DANGKVAFDG--------LELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIA 434
Query: 572 -------GKADNRNANALLDLQTKSTVGTNSGTGTSFTSAYASLVERVGAKASQATIDTT 624
G +DNRN ALLDLQ+ S G SF AYASLV +G K + +T
Sbjct: 435 MASEEDAGDSDNRNGQALLDLQSNSKT---VGGAKSFNDAYASLVSDIGNKTATLKTSST 491
Query: 625 ATQAVLKSATESRSAVSGVNLDDEAASLVKFQHYYTASSQIIKAAQETFSTLIN 678
V+ + + ++SGVNLD+E +L +FQ YY A++Q+++ A F LIN
Sbjct: 492 TQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 545