Pairwise Alignments

Query, 776 a.a., DNA ligase from Pseudomonas putida KT2440

Subject, 566 a.a., DNA ligase B from Pseudomonas putida KT2440

 Score =  145 bits (365), Expect = 8e-39
 Identities = 154/591 (26%), Positives = 256/591 (43%), Gaps = 67/591 (11%)

Query: 3   AETRILELRAELDQHNYRYYVLDEPSVPDAEYDRLFNELKALEAEHPHLVTPDSPTQRVG 62
           A+  + +LRA L + +  Y+      V D  YD+    L  L+       +P SP     
Sbjct: 31  AKAEVAQLRATLARWDEHYHRQGIALVADELYDQSHERLNHLQQCFAVGTSP-SPLASAR 89

Query: 63  GAALAAFSQVRHEVPMLSLGNAFEEADLREFGRRVVEGLDQPGAVDYSCEPKLDGLAVSL 122
           G        V H VP   +      AD +   R +       G      +PK+DG+AVSL
Sbjct: 90  GP-------VPHPVPHTGVDKL---ADRQAVARWMT------GKTGVWVQPKVDGVAVSL 133

Query: 123 LYRDGQLVQGATRGDGTTGEDISANVRTVRNIPLKLQGKGWPAVLEVRGEVYMSKAGFDR 182
            Y+ G+LVQ  +RGDG  G D S ++  +  +  +L        L ++GE+Y+      R
Sbjct: 134 TYQQGRLVQLTSRGDGVHGHDWSRHIPQLGAVTRQLPEA---VDLHLQGELYL------R 184

Query: 183 LNA-AQAEAGGKTFANPRNAAAGSLRQLDSKITASRPLEFCCYGVGQVSESIGDSHIGIL 241
           L+   QA+AG    AN R   AG L +          +    +G     E   +     L
Sbjct: 185 LDEHVQAKAGS---ANARGTVAGLLARKQLTGAQGNAIGLFVWGWPHGPEQQAER----L 237

Query: 242 EQLKAWGLPISRELKHAAGIEECLAYYRDIGERRNSLPYEIDGVVFKVNSLAAQRELGFR 301
            QL   G P S+    A    E  A++R+   R ++LP+  DGV+ +  S        ++
Sbjct: 238 AQLARLGFPDSQLYSIAIDTLEDAAHWREHWYR-SALPFATDGVILRQGSRPPAER--WQ 294

Query: 302 AREPRWAIAHKFPAMEELTEVLDVEFQVGRTGAVTPVARLKPVKVAGVTVSNATLHNMDE 361
           A+ P W  A K+P  + L EV DV F+VGRTG VTPV  ++PV +    ++  +L ++  
Sbjct: 295 AKAPYWIAAWKYPYAQALAEVRDVRFRVGRTGRVTPVLHVQPVTLDDRRITQVSLGSLAR 354

Query: 362 IARLGLRIGDTVIIRRAGDVIPQVMQVVLERRPQGARPVEVPSACPVCGSQVERTRLVKR 421
             RL +R GD V I  AG  IP++  VV   R    +P+  P+       Q       + 
Sbjct: 355 WQRLDIRPGDQVAISLAGLTIPRLEHVV--HRAVERQPITAPAP-----DQHHAHSCWQA 407

Query: 422 SKGKETTSEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIDGLGEKSVEQLVDEGLIGSP 481
           S+G                 C  Q    +     ++ + +   G  +  +LV+ GL+ S 
Sbjct: 408 SEG-----------------CDEQFIARLTWLGGKQGLALPRTGPGTWRRLVEAGLVTSM 450

Query: 482 ADLYKLQFEQVVGLEGFAEVSTQKLLDAIEASKRPSLARFIYALGIPDVGEETAKVLARS 541
            D  +L  E++    G + ++  ++L + + ++     +++ ALG+P       K L  +
Sbjct: 451 TDWLQLDAERLQQAPGISRLTATQMLGSFDQARSRPFDQWLRALGVP-----IGKHLPLT 505

Query: 542 LGSLARVQQALPQVLTYLPDIGLEVAYEIHNFFEDEHNQKVIQQLLDSGMQ 592
               A   ++  Q  T +P IG + + ++  FF     Q +  QL ++G++
Sbjct: 506 GNWQALASRSAGQWQT-VPGIGAKRSRQLVEFFAASEVQAIAAQLAEAGIE 555