Pairwise Alignments

Query, 540 a.a., AMP-binding domain protein from Pseudomonas putida KT2440

Subject, 560 a.a., Medium-chain-fatty-acid CoA ligase from Pseudomonas putida KT2440

 Score =  271 bits (692), Expect = 6e-77
 Identities = 173/519 (33%), Positives = 267/519 (51%), Gaps = 33/519 (6%)

Query: 37  VIHGAIRRNWQETYQRCRRLASALAGRGIGRGDTVAVMLPNTPTMLEAHFGVPMTGAVLN 96
           V    +R  + +  +R  RLA+ L   G+  GDTVAVM  ++   LE  F +PM GAV++
Sbjct: 37  VYRDQMRLTYPQLNERIARLANVLTEAGVKAGDTVAVMDWDSHRYLECMFAIPMIGAVVH 96

Query: 97  TLNVRLDAEAIAFMLQHGEAKVLITDREF---HAVIEGALALLEHPPLVVDVDDPEYGEG 153
           T+NVRL  E I + + H E +V++ + +F   +  I G L  ++   L+ D  D      
Sbjct: 97  TINVRLSPEQILYTMNHAEDRVVLVNSDFVGLYQAIAGQLTTVDKTLLLTDGPDKTAELP 156

Query: 154 RAVSQLDYEALLNEGDPEFAWEWPD-DEWQAISLNYTSGTTGNPKGVVYHHRGAYLNALG 212
             V +  YE LL    P +  ++PD DE    +  YT+GTTGNPKGV + HR   L+ L 
Sbjct: 157 GLVGE--YEQLLAAASPRY--DFPDFDENSVATTFYTTGTTGNPKGVYFSHRQLVLHTLA 212

Query: 213 NQMTWA-------MGHRPVYLWTLPMFHCNGWCYPWTITALAGTHVFLRRVDPQKILTLI 265
                        +G   VY+   PMFH + W  P+  T L    V+  R +P  ++ L 
Sbjct: 213 EASVTGSIDSVRLLGSNDVYMPITPMFHVHAWGIPYAATMLGMKQVYPGRYEPDMLVKLW 272

Query: 266 REHKVSHLCGAPIVLNALVNMPEAAKAAIEHPVQAMVAGAAPPAKVIGAVEQMGIKVTHT 325
           RE KV+     P +L  L+N P A         + ++ G++    +  A    GI++T  
Sbjct: 273 REEKVTFSHCVPTILQMLLNCPNAQGQDFGG-WKIIIGGSSLNRSLYQAALARGIQLTAA 331

Query: 326 YGLTEVYGPVTVCAWHDEWDALSLEERARIKSRQGVRYPTLDGLMVADPQTLQPVPRDGD 385
           YG++E    ++    +DE  A S +ER   + + GV  P ++  +V        +P DG+
Sbjct: 332 YGMSETCPLISAAHLNDELQAGSEDERVTYRIKAGVPVPLVEAAIVDGEGNF--LPADGE 389

Query: 386 TLGEIFMRGNTVMKGYLKNPEATAEAFRGGWFHTGDLAVWHADGYIEIKDRLKDIIISGG 445
           T GE+ +R   +  GY K PE + E ++GGW HTGD+A     GYI+I+DR+KD+I +GG
Sbjct: 390 TQGELVLRAPWLTMGYFKEPEKSEELWQGGWLHTGDVATLDGMGYIDIRDRIKDVIKTGG 449

Query: 446 ENISTIEVEDALYKHSAVLEAAVVARPDEKWGETPCAFVALKPGREDTREADITSWCREH 505
           E +S++++ED + +H AV E AVV   D +WGE P A +  + G +   +A      +EH
Sbjct: 450 EWVSSLDLEDLISRHPAVREVAVVGVADPQWGERPFALLVARDGHDIDAKA-----LKEH 504

Query: 506 LAGF---------KVPKTV-VFGELPKTSTGKIQKYVLR 534
           L  F          +P  + +  E+PKTS GK+ K  +R
Sbjct: 505 LKPFVEQGHINKWAIPSQIALVTEIPKTSVGKLDKKRIR 543