Pairwise Alignments

Query, 562 a.a., Butyrate--CoA ligase from Pseudomonas putida KT2440

Subject, 562 a.a., long-chain fatty acid--CoA ligase from Pseudomonas simiae WCS417

 Score =  262 bits (669), Expect = 3e-74
 Identities = 168/545 (30%), Positives = 268/545 (49%), Gaps = 26/545 (4%)

Query: 22  DAFDATVARFPDREALVVHHQALRYTWRQLADAVDQHARALMA-LGVQPGDRLGIWAPNC 80
           + F+ +   F DR A    +  +  T+ +L       A  L     ++PGDR+ +  PN 
Sbjct: 28  EVFERSCKAFADRPAF--SNMGITLTYAELERQSAAFAGYLQQHTDLKPGDRIAVQMPNV 85

Query: 81  AEWCITQFASAKVGAILVNINPAYRSSELDYALGQSGCRWVICADAFKTSDYHAMLQGLL 140
             + I  F + + G I+VN NP Y   E+ +    +G R ++  + F +          +
Sbjct: 86  LHYPIAVFGALRAGLIVVNTNPLYTPREMRHQFKDAGVRALVYLNLFGSRVQEVCSDTEI 145

Query: 141 PGLASSQPGALICERFPELRG-----VVSLALSPPPGFLAWHALQARAEVVSGEALAARQ 195
             L  ++ G  +    P  +G     +V       P +    A+  +  +  G  L   +
Sbjct: 146 DYLIEARMGDFM----PTAKGWLVNTLVDKVKKMVPAYSLPRAVAFKRALHMGAGLGVTR 201

Query: 196 AQLRCDDPINIQYTSGTTGFPKGATLSHSNILNNGYMVGESLGLTEHDR----------L 245
             +  DD   +QYT GTTG  KGA L+H N++ N   V   +  T  D           +
Sbjct: 202 HPVTLDDIAVLQYTGGTTGLAKGAMLTHGNLVANMQQVRACMSQTGDDGHPLIKEGQEVM 261

Query: 246 VVPVPLYHCFGMVMANLGCMTHGSALIYPSDAFDPLATLRAVAQEKATALYGVPTMFIAE 305
           + P+PLYH +      +  M  G+  +  ++  D    ++ + + + T L G+ T+F+A 
Sbjct: 262 IAPLPLYHIYAFTANCMCMMVSGNHNVLITNPRDIGGFIKELKKWRFTGLLGLNTLFVAL 321

Query: 306 LDHPQRGEFDLSSLRTGIMAGATCPIEVMRRVIGEMHMAEVQIAYGMTETSPVSLQTGAA 365
           +DHP     D S L+    +G T  I+       ++    +   YG+TETSPV+      
Sbjct: 322 MDHPDFKSLDFSHLKL-TNSGGTALIKATAERWQQITGCAIGEGYGLTETSPVASTNPYG 380

Query: 366 DDLERRVTSVGRTQPRLESKVVDAEGNTVPRGEIGELCTRGYSVMLGYWNNPKATAESID 425
           +  + R+ +VG   P    KV+D  G  +P GE GELC +G  VM GYW  P+ATAE++D
Sbjct: 381 N--KSRLGTVGIPVPATAMKVIDDHGVELPLGERGELCIKGPQVMKGYWQQPEATAEALD 438

Query: 426 AEGWMHTGDLAVMDEQGYVRIVGRSKDMIIRGGENIYPRELEEFFFTHPAVADVQVIGVP 485
           AEGW+ TGD+AV+DE+G+V IV R KD+II  G N+YP E+E+    HPAVA+  VIGVP
Sbjct: 439 AEGWLKTGDIAVIDEEGFVSIVDRKKDLIIVSGFNVYPNEIEDVVMAHPAVANCAVIGVP 498

Query: 486 CSKYGEEIVAWVRLHPGHAVSEVELREWARARIAHFKVPRYFRFVDEFPMTVTGKVQKFR 545
             + GE +  +V +     VS  EL+ + +     +KVP++    D  PMT  GK+ +  
Sbjct: 499 DERTGEAVKLFV-VARAEGVSLEELKAYCKTNFTGYKVPKHIVLRDSLPMTPVGKILRRE 557

Query: 546 MREIS 550
           +R+I+
Sbjct: 558 LRDIA 562