Pairwise Alignments

Query, 627 a.a., p-coumarate CoA-ligase (EC 6.2.1.12) (from data) from Pseudomonas putida KT2440

Subject, 618 a.a., Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) from Variovorax sp. SCN45

 Score =  126 bits (316), Expect = 3e-33
 Identities = 173/661 (26%), Positives = 261/661 (39%), Gaps = 154/661 (23%)

Query: 47  PLPARLLERLVHWAQVRPDTTFIAARQAD-GAWRSISYVQMLADVRTIAANLLGLGLSAE 105
           PLP +LL    HWA  RPD   +A RQ + G W+ IS+       R     LL LGL   
Sbjct: 17  PLPQQLL----HWAARRPDA--VALRQKEYGVWQPISWSAYAQQARWFGLGLLRLGLRPG 70

Query: 106 RPLALLSGNDIEHLQIALGA-----MYAGIAYCPVSPAYALLSQDFAKLRHVCEVLTPGV 160
           + +A+LS N  E +   LGA     + AG+   P SPA         ++ H+  +    V
Sbjct: 71  QAVAVLSENRKEWVFAQLGAALVRGVTAGVY--PTSPA--------EEIEHLLALSDAPV 120

Query: 161 VFVSDSQPFQRAFEA----------VLDDSVGVISVRGQVAGRPHISFDSLLQPGDLAAA 210
           +   D +   +              VL DS G+   R    G   I FD ++  G    A
Sbjct: 121 IVCEDQEQLDKVLSIRDRLPHLHTIVLIDSRGLR--RYDRTGM--IDFDEVVALGRRFEA 176

Query: 211 D----AAFAATGP--DTIAKFLFTSGSTKLPKAVITTQRMLCANQQMLLQTFPTFAEEPP 264
           D    AA A  GP  D I   +FTSGST  PKA + + R L    + L         E  
Sbjct: 177 DEPAAAAAATVGPALDDIGLIVFTSGSTGRPKAAMMSWRGLGTAARGLNSVLG--CREGD 234

Query: 265 VLVDWLPWNHTFGGSHNLGIVLYNGGSFYLDAGKPTPQGFAETLR----NLREISPTAYL 320
            LV +LP  H       +G  ++   S ++         FAE+LR    +LRE+SP  + 
Sbjct: 235 SLVSYLPLCH-------MGEQMF---SLHIPVAFGAVVNFAESLRTVQDDLRELSPRIFF 284

Query: 321 TVPKGWEELVKALEQDPALREVFFARIKLFFFAA------AGLSQSVWDRLDR------- 367
            VP+ WE+   A++    LRE    R+ L+  A       AG+ ++ W  L R       
Sbjct: 285 GVPRIWEKFHAAIQN--KLREAGGLRLWLYERAMRSLAHDAGVPRAQWSWLQRARWAVWY 342

Query: 368 ----------IAEQHCGERIRMMAGL-------------------GMTEASPSCTFTTGP 398
                     +  + C   +   A +                   GMTEAS + T   G 
Sbjct: 343 VLILRSLLNYLGLRGCRVAVSAAAPIAPEVLRFFRVLGVPIREAYGMTEASGATTMQPGD 402

Query: 399 LSMAGYVGLPAPGCEVKLVPVGDKLEARFRGPHIMPGYWRSPQQTAEAFDEEGFYCSGDA 458
            S  G VG+P PG EV+L   G+ L    RG  +  GY ++ + T EA D EG+  +GD 
Sbjct: 403 DSPVGTVGVPYPGVEVQLADDGEIL---IRGEVVFRGYLKNDEATREAIDAEGWLHTGDI 459

Query: 459 LKLADARQPELGLMFDGRIAE---DFKLSSGVFVSVGPLRNRAVLEGSPYVQDIVVTAPD 515
            +  D+         + RI +   D  +++G   ++ P      +  SP++++ V  A  
Sbjct: 460 GRWEDSPSKNAPAGRELRIVDRKKDIMITAG-GKNITPSEIENAMRFSPFIKEAVAIADR 518

Query: 516 RECLGLLV-----------------------FPRLPECRRLAGLAEDASDARVLANDTVR 552
           R  +  L+                          LP+ R L     D ++AR+     VR
Sbjct: 519 RHFVSALIQIDFETVSKWSEEKGLVYTTFRSLVELPQVRELVQREVDTANARMPQVQQVR 578

Query: 553 SWFADWLERLNRDAQGNASRIEWLSLLAEPPSIDAGEITDKGSINQRAVLQRRAAQVEAL 612
            +                       LL +    D GE+T    + ++AV ++ A  +E +
Sbjct: 579 KF----------------------HLLTKELDHDDGEVTATMKVRRKAVFEKYAQVIEEI 616

Query: 613 Y 613
           Y
Sbjct: 617 Y 617