Pairwise Alignments

Query, 627 a.a., p-coumarate CoA-ligase (EC 6.2.1.12) (from data) from Pseudomonas putida KT2440

Subject, 603 a.a., Aromatic--CoA ligase from Variovorax sp. SCN45

 Score =  441 bits (1134), Expect = e-128
 Identities = 247/588 (42%), Positives = 351/588 (59%), Gaps = 17/588 (2%)

Query: 28  VQVSHVDDVLRMQPVEPLAPLPARLLERLVHWAQVRPDTTFIAARQADG-AWRSISYVQM 86
           V++   D    ++    L P    + E +  WA+  PD   +A R   G  WR + Y  +
Sbjct: 17  VRIDFDDGSFALRSPVALKPYARCIGEWIERWARETPDALALAERDETGEGWRKLDYRAL 76

Query: 87  LADVRTIAANLLGLGLSAERPLALLSGNDIEHLQIALGAMYAGIAYCPVSPAYALLSQDF 146
              V  +A  LL + L A +P+ +LS N I+H  + L AM+ G   C +S AY+ +++D 
Sbjct: 77  RQAVGAVAQALLDMNLPAGQPVVILSDNAIDHAVLMLAAMHIGRTACSLSSAYSRMAKDT 136

Query: 147 AKLRHVCEVLTPGVVFVSDSQPFQRAFEAVLDDSVGVISVRGQVAGRPHISFDSLLQPGD 206
           ++L  + + L P +++ SD++ +  +      ++  V S R   A    + F+ LL   +
Sbjct: 137 SRLHGMLQALKPALIYASDAKVYGGSLAGCGVEAATVFS-RNADAHPGAVPFERLLAVKE 195

Query: 207 LAAADAAFAATGPDTIAKFLFTSGSTKLPKAVITTQRMLCANQQMLLQTFPTFAEEPPVL 266
             A   AFAA  PDT AK+L TSGST  PK V+ T RMLCANQQM+ QT+   A E PVL
Sbjct: 196 TPAVMQAFAAVLPDTHAKYLLTSGSTGKPKVVVNTHRMLCANQQMMAQTWRFLAREAPVL 255

Query: 267 VDWLPWNHTFGGSHNLGIVLYNGGSFYLDAGKPTPQGFAETLRNLREISPTAYLTVPKGW 326
           VDWLPW+HTFGG+HNL +VL +GG+ Y+D G+P P    +T+RNLRE+ PT    VP+G+
Sbjct: 256 VDWLPWSHTFGGNHNLHMVLCHGGALYIDEGRPAPGLIEKTVRNLREVKPTLLFNVPRGF 315

Query: 327 EELVKALEQDPALREVFFARIKLFFFAAAGLSQSVWDRLDRIAEQHCGER-IRMMAGLGM 385
           + L+  LE D AL     +R++L F+AAA L+ S W RL+ +A++    R + +    G 
Sbjct: 316 DMLLPYLEADDALAAEVLSRLRLAFYAAAALAPSTWQRLEAVAKRVRPSRPLWLTTSWGA 375

Query: 386 TEASPSCTFTTGPLSMAGYVGLPAPGCEVKLVPVGDKLEARFRGPHIMPGYWRSPQQTAE 445
           TE SP+ T     L  AG +G P PG E+K VP G+KLE R +G  + PGY  +P++TA+
Sbjct: 376 TETSPAITSAHWKLDGAGCIGAPLPGLELKFVPNGEKLEMRVKGVSVFPGYRDAPRETAQ 435

Query: 446 AFDEEGFYCSGDALKLADARQPELGLMFDGRIAEDFKLSSGVFVSVGPLRNRAVLEGSPY 505
           AFDEEG+Y  GDA  LAD+ QP  G++F+GR+AEDFKLSSG +VSVG LR + V   +P+
Sbjct: 436 AFDEEGYYRIGDAGFLADSAQPAQGVIFNGRVAEDFKLSSGTWVSVGTLRVKLVSMLAPH 495

Query: 506 VQDIVVTAPDRECLGLLVFPRLPECRRLAGLAEDASDARVLANDTVRSWFADWLERLNRD 565
            QD+V+T  DR+ +G+LVFP              +  A  L  + +    A  L  L  +
Sbjct: 496 AQDVVLTGHDRDEIGMLVFP--------------SPQAAALPAEALHERIAGMLRALRDE 541

Query: 566 AQGNASRIEWLSLLAEPPSIDAGEITDKGSINQRAVLQRRAAQVEALY 613
             G++       +LAEPPS+DAGEITDKG INQRAVL RRAA+V+ L+
Sbjct: 542 GAGSSQCPACALVLAEPPSLDAGEITDKGYINQRAVLARRAAEVDRLH 589