Pairwise Alignments

Query, 318 a.a., tRNA (mo5U34)-methyltransferase from Pseudomonas putida KT2440

Subject, 323 a.a., predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) from Escherichia coli BW25113

 Score =  354 bits (909), Expect = e-102
 Identities = 178/324 (54%), Positives = 232/324 (71%), Gaps = 8/324 (2%)

Query: 1   MIDLSPLVRRLAGTPLASWSQGLQAQL-DAKLEKGHGDLDRWRGALEALPALQPSEIDLV 59
           MID       +A   L+ W + L AQ+ + + E+ HG   +W  A+E LP ++P  +DL+
Sbjct: 1   MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL 60

Query: 60  NGLRLDCDCDDATRAQMRQA---LMGLSPWRKGPFDLFGVHVDTEWRSDWKWSRVGPHL- 115
           + +  + + +  +  Q+++    +  L PWRKGPF L+GV++DTEWRSDWKW RV PHL 
Sbjct: 61  HSVTAESE-EPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLS 119

Query: 116 DLQGKRVLDVGCGNGYYQWRMLGAGADMVIGVDPNWLFFCQFQAVQQYLPELPAWHL-PF 174
           DL G+ +LDVGCG+GY+ WRM+GAGA + +G+DP  LF CQF+AV++ L      HL P 
Sbjct: 120 DLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPL 179

Query: 175 ALEDLPANLEGFDTVFSMGVFYHRRSPIEHLLALKDCLVKGGELVLETLVIEGDENQVLV 234
            +E LPA L+ FDTVFSMGV YHRRSP+EHL  LKD LV  GELVLETLVI+GDEN VLV
Sbjct: 180 GIEQLPA-LKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLV 238

Query: 235 PEDRYAQMRNVWYLPSVPALARWLRRAGFSDVRCVDVSVTSVEEQRSTDWMRYQSLSDFL 294
           P DRYAQMRNV+++PS  AL  WL++ GF D+R  DVSVT+ EEQR T+WM  +SL+DFL
Sbjct: 239 PGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFL 298

Query: 295 DPNDHSKTVEGLPAPRRATLLARK 318
           DP+D  KTVEG PAP+RA L+ARK
Sbjct: 299 DPHDPGKTVEGYPAPKRAVLIARK 322