Pairwise Alignments

Query, 679 a.a., Methionine--tRNA ligase from Pseudomonas putida KT2440

Subject, 698 a.a., Methionyl-tRNA synthetase (EC 6.1.1.10) from Variovorax sp. SCN45

 Score =  677 bits (1747), Expect = 0.0
 Identities = 369/704 (52%), Positives = 463/704 (65%), Gaps = 31/704 (4%)

Query: 1   MSEPRQILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQCIYVCADDAHGSAIML 60
           MS PR++ VT+ALPYANG  H+GHM+EYIQ D+WVR Q++ G    +VCADDAHG+AI +
Sbjct: 1   MSAPRKLFVTTALPYANGKFHIGHMMEYIQADIWVRNQRMNGADVNFVCADDAHGAAITI 60

Query: 61  RAEKEGITPEQLIANVQAEHSSDFADFLVDFDNFHSTHSDENRELSSLIYSRLREAGHIA 120
            A+K G+TP+  +A + A        + + FDN+HST + EN +L+  IY  LR  G IA
Sbjct: 61  AADKAGVTPQAFVAEIAAGRKPYLDGYHIAFDNWHSTDAPENHQLAQDIYRDLRANGLIA 120

Query: 121 TRSVTQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTELKNPKSAISGA 180
           T+SV Q++DP+KGMFLADRFIKG CP C ++DQYGDNCE CGA YAPTEL NP SA+SGA
Sbjct: 121 TKSVEQFYDPQKGMFLADRFIKGECPNCHSKDQYGDNCEVCGAVYAPTELINPYSALSGA 180

Query: 181 TPVLRDSQHFFFKL--PDFQAMLQQWTRS-GTLQDAVANKLAEWL------DSGLQEWDI 231
            P LR S+HFFFKL  P  +A L++WT + G +Q  V NK+ EWL        GL +WDI
Sbjct: 181 KPELRSSEHFFFKLSDPRCEAYLKEWTAAPGHVQPEVQNKIREWLYKDDEGKGGLGDWDI 240

Query: 232 SRDAPYFGFEIPGEPGKYFYVWLDAPIGYMASFKNLCARR-PELDFDAF----WHEGSKA 286
           SRDAPYFG EIP  PGKYFYVWLDAP+GY+AS KNL  +R  EL  D      +    + 
Sbjct: 241 SRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKRCIELGGDGISYTEYMADPEL 300

Query: 287 ELYHFIGKDIVNFHALFWPAMLEGAGFRKPTAVNVHGYLTVNGAKMSKSRGTFIKARTYL 346
           E  HFIGKDI+ FH LFWPAML  +G + P AV VHG+LTV+G KMSKSRGT I    YL
Sbjct: 301 EQVHFIGKDIITFHTLFWPAMLHFSGRKAPNAVYVHGHLTVSGEKMSKSRGTGIDPLKYL 360

Query: 347 D-HLQPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNEG 405
              L PE+LRYY AAKL    +D+D N EDFV +VNSDLVGK +NIASR AGFI K   G
Sbjct: 361 SIGLDPEWLRYYIAAKLNGRNEDIDFNPEDFVARVNSDLVGKYINIASRAAGFIGKRFGG 420

Query: 406 ----VMVGGDAAPELTEAFLAAAPSIAEAYEARDFGRAMREIMALADRANAWIADKAPWS 461
               V   GDA   L      AA  I   Y+ RD+ RA+RE+MALAD+ N ++    PW 
Sbjct: 421 KLGEVSADGDA---LLFTLRDAAQQIHSLYDGRDYARALREVMALADKVNEYVDQNKPWE 477

Query: 462 LAKQEGKQDEVQAICAQGINLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHLTRL-E 520
           LAK+EG +  +  +C   I  FR L ++LKPVLP LA + EAFL  +PL W D    L  
Sbjct: 478 LAKKEGAEARLHDVCTVCIEAFRLLTLYLKPVLPALALNVEAFLKTSPLVWADATQSLPA 537

Query: 521 NHQLNPFKALMSRIEPAKVEAMVAASKEDLLAAEAKAPAGNGELAKDPLSAEIEFDTFAA 580
            H +  +K LM R +P +V+ +  A   +  AA A A         + ++  I  D FA 
Sbjct: 538 GHAIGDYKHLMQRADPKQVDKLFDA--PEAAAAPAVATQAQELPGGEAIAPTITIDDFAK 595

Query: 581 VDLRVALIVKAEAVAGADKLLQLTLDIGD-ERRNVFSGIKSAYPDPSKLEGRLTMMVANL 639
           +DLR+A IV  E V G+ KLL+LTLD G+ + RNVFSGI SAY  P +L G+LT++VANL
Sbjct: 596 IDLRIAKIVACEKVEGSTKLLRLTLDAGEGKTRNVFSGIASAY-QPEQLVGKLTVLVANL 654

Query: 640 KPRKMRFGVSEGMVMAAGPGGEE----IYLLSPDSGAKPGQRIK 679
            PRKM+FGVSEGMV+AA  G E+    I++L P  GA PG R++
Sbjct: 655 APRKMKFGVSEGMVLAASHGDEKAHPGIHVLEPWPGATPGMRVR 698