Pairwise Alignments
Query, 1434 a.a., putative ATP-dependent DNA helicase lhr from Pseudomonas putida KT2440
Subject, 1538 a.a., ATP-dependent RNA helicase SrmB from Escherichia coli HS(pFamp)R (ATCC 700891)
Score = 591 bits (1524), Expect = e-172
Identities = 475/1494 (31%), Positives = 701/1494 (46%), Gaps = 182/1494 (12%)
Query: 12 ELFHPAVSTWFRRHFATVTDAQAQAWPLIHAGQSMLLAAPTGSGKTLSAFLAVLDELLRE 71
++F PA WF R F T Q Q W + + L+ APTGSGKTL+AFL LD L RE
Sbjct: 13 DVFSPATRDWFLRAFKQPTAVQPQTWHVAARSEHALVIAPTGSGKTLAAFLYALDRLFRE 72
Query: 72 GMKHQGELPAQ--TLVIYVSPLKALSNDIRLNLQAPLEGISQAIAEQGLNAPRITTAVRT 129
G + E + + ++Y+SP+KAL D++ NLQ PL+GI+ +G + +RT
Sbjct: 73 GGEDTREAHKRKTSRILYISPIKALGTDVQRNLQIPLKGIADERRRRGETEVNLRVGIRT 132
Query: 130 GDTPQKERAAMRKLAPHILVTTPESLYVLMGSASGREGLAHVHTVIVDEIHALAGNKRGA 189
GDTP +ER+ + + P IL+TTPESLY+++ S + RE L V TVI+DE+HA+AG+KRGA
Sbjct: 133 GDTPAQERSKLTRNPPDILITTPESLYLMLTSRA-RETLRGVETVIIDEVHAVAGSKRGA 191
Query: 190 HLALTLERLQALCKKPLRRIGLSATQRPVERVAQFLVGSGRPCAIVDIGHARKRDLAIEV 249
HLAL+LERL AL +RIGLSAT R VA FL G RP +V+ R + I V
Sbjct: 192 HLALSLERLDALLHTSAQRIGLSATVRSASDVAAFL-GGDRPVTVVNPPAMRHPQIRIVV 250
Query: 250 PPVPLGAVMAI--------------DVWGLVYDRLATLAREHRTTLVFVNTRRLAERITR 295
P + V ++ +W + + HR+T+VF N+R LAE++T
Sbjct: 251 PVANMDDVSSVASGTGEDSHAGREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTA 310
Query: 296 HLSD----RLGKEAVAA---------------------------HHGSLSKALRLDAEQR 324
L++ RL + A HHGS+SK R EQ
Sbjct: 311 RLNELYAARLQRSPSIAVDAAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQA 370
Query: 325 LKSGQLQVLVATASLELGIDIGDIDLVCQVASPGSIAAFLQRVGRAGHQVDGVPKGRLFP 384
LKSG+L+ +VAT+SLELGID+G +DLV QVA+P S+A+ LQR+GRAGHQV GV KG FP
Sbjct: 371 LKSGELRCVVATSSLELGIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFP 430
Query: 385 TSRDDLIECVALLDCVRQGELDALHIPRAPLDVLAQQIVAEASNQAWHEQELLDCLRQAT 444
+R DL++ +++C+ G L+ L P PLDVLAQQ VA A+ A E +R+A
Sbjct: 431 RTRRDLVDSAVIVECMFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAA 490
Query: 445 PYAELDQHHYQALLRMLAEGY-NGRQGVRSAYLHRDAVSGTLRGRRGSQLTALTSGGTIP 503
P+ +L + + A L ML+ Y +G L + +G L R G+QL A+TSGGTIP
Sbjct: 491 PWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIP 550
Query: 504 DNADYAVLL-----EPQALNIGSVNEDFAVESIAGDIFQLGNASYRILRVEPGRVRVEDA 558
D Y+VLL + + +G ++E+ ES DI LG S+RI ++ +V V A
Sbjct: 551 DRGMYSVLLPEGEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTLA 610
Query: 559 HGLPPTIPFWLGEAPGRSDELSAAVARLQARIDQQLALAGGDTVEVLAWLQATFELDEAC 618
G +PFW GE GR EL + + +G + WL +E
Sbjct: 611 PGRSARLPFWRGEGNGRPAELGEMIGDFLHLLADGAFFSG----TIPPWL-----AEENT 661
Query: 619 AGQLLDYLARTREVLGALPSQDTLVMERFFDESGGTQLIIHSPYGSRINRAWGLALRKRF 678
+ + R G +P LV+ER DE G ++I+HSPYG R++ W LA+ R
Sbjct: 662 IANIQGLIEEQRNATGIVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRI 721
Query: 679 CRTFNFELQAAASEDAIVLSLSTSHSFELDEVWRYLNSRSAEPILIQALLDAPLFGVRWR 738
+ + AS+D IV + + D I+ +A+ + LF R+R
Sbjct: 722 HALWGADASVVASDDGIVARIPDTDGKLPDAAIFLFEPEKLLQIVRKAVGSSALFAARFR 781
Query: 739 WNAGVAMALPRFVGGRKVAPQIQRMKSEDLVAAVFPDQIACLENIAGERQIPDHPLVEQT 798
A A+ +P G + QR+++ L+ +IA + PD P++ +T
Sbjct: 782 ECAARALLMPGRTPGHRTPLWQQRLRASQLL------EIA--------QGYPDFPVILET 827
Query: 799 LDDCLHEAMDSEGWLALLRRMESGAVRLLCRDLPAPSPLASAIL--NARPYAFLDDAPLE 856
L +CL + D L+RR+ G +++ PSP A+++L + + DAPL
Sbjct: 828 LRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLA 887
Query: 857 ERRTQAV---LNRRWNDVHSSDDLGALDADAIAAVAAE----AWPQPANPDE-MHEALMS 908
ERR + N + D LD I V E A + A +E + + L
Sbjct: 888 ERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRE 947
Query: 909 LGAI----------GQVEVDANPCWDLL-----LRQLAKAGRALRLPDDKLWLARERLNL 953
LG + G E A+ +LL + L DD L R+ L +
Sbjct: 948 LGPMTVEDLAQRHTGSSEEVASYLENLLAVKRIFPAMISGQERLACMDDAARL-RDALGV 1006
Query: 954 LLALYPTAHREPALERLAGFDQPIEPDHALSELLRARLSGHGPQTLAQIAAPLGKPVTDI 1013
L P + E L R++ + L +L L H T Q+A + +
Sbjct: 1007 RL---PESLPEIYLHRVS---------YPLRDLFLRYLRAHALVTAEQLAHEFSLGIAIV 1054
Query: 1014 EQALARLEAEGYVLRGHFSPGATDLQ---WCERHLLARIHRYTVKRLRREIEPVSLQDFM 1070
E+ L +L +G V+ +LQ W + R+ +++ R PV+ +
Sbjct: 1055 EEQLQQLREQGLVM---------NLQQDIWVSDEVFRRLRLRSLQAAREATRPVAATTYA 1105
Query: 1071 RFLFDWQHLAPDER-------------LRGPQAVAEVLGQLQGFPSAAAAWEAELLPARI 1117
R L + Q + P G V V+ QL G A+ WE+++LPAR+
Sbjct: 1106 RLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPASLWESQILPARV 1165
Query: 1118 KDYSPHWLDEACRSGQFAWSRLAATVASSTLTSTPLVLLPRDQLNTWRSLAPAPAVDALG 1177
+DYS LDE +G WS L + L Q S PA A A
Sbjct: 1166 RDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHL------QEYAAESFTPAEADQANR 1219
Query: 1178 LRAQRVHEALKTQGALFF---------DELAHEAHLLPSELETALQELVGSGLARADSFT 1228
Q+ A+ G +F D++ L S L+ AL LV G+ +D +
Sbjct: 1220 SALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDL--SALQEALWALVWQGVITSDIWA 1277
Query: 1229 GLRSLITPATK-----RSSRNSRRGHP----PLSSSMAH-----AGRWALLRRSDAPPDH 1274
LR+L ++ R S +RRG P P+S +++ AGRW+LL+ P +
Sbjct: 1278 PLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPNLAGRWSLLQVE--PLND 1335
Query: 1275 DQRLEHIARTLLRRYGVICWRLLARES--DVLPPWRELLRCYHRLEARGEIRGGRFIAGL 1332
+R+ +A +L RYG+I + + E+ P + L R +E G I GRF+ GL
Sbjct: 1336 TERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCR---SMEDSGRIMRGRFVEGL 1392
Query: 1333 AGEQFALPEAVVLLRQVRRREPDG---TLQVVSAGDPLNLIGSLLPGAKVPAVI 1383
G QFA + LR + + T +SA DP N+ G+LLP PA +
Sbjct: 1393 GGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNLLPWPAHPATL 1446
Score = 29.3 bits (64), Expect = 0.003
Identities = 17/51 (33%), Positives = 23/51 (45%)
Query: 1141 ATVASSTLTSTPLVLLPRDQLNTWRSLAPAPAVDALGLRAQRVHEALKTQG 1191
AT A+ T TP+ L D N W +L P PA A + +R + G
Sbjct: 1410 ATQATQTRHYTPVALSANDPANVWGNLLPWPAHPATLVPTRRAGALVVVSG 1460