Pairwise Alignments

Query, 560 a.a., Medium-chain-fatty-acid CoA ligase from Pseudomonas putida KT2440

Subject, 541 a.a., 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type from Variovorax sp. SCN45

 Score =  325 bits (834), Expect = 2e-93
 Identities = 191/540 (35%), Positives = 297/540 (55%), Gaps = 29/540 (5%)

Query: 17  PLLIKRLLMSGSRYEKTREIVYRDQM----RLTYPQLNERIARLANVLTEAGVKAGDTVA 72
           PLL+  L+   +RY     +V  +        T+ Q+     RL++ L + GV++G+  A
Sbjct: 4   PLLVSSLIAHAARYHADTPVVSVNTTGGIEHTTWGQVEINARRLSSALAKLGVQSGERCA 63

Query: 73  VMDWDSHRYLECMFAIPMIGAVVHTINVRLSPEQILYTMNHAEDRVVLVNSDFVGLYQAI 132
            + W++ R+LE  + +   G V HT+N RLS E ++Y +NHA+D+V+ ++  F+ +   +
Sbjct: 64  TIAWNNRRHLEIYYGVSGAGMVCHTVNPRLSAETLIYIVNHADDQVLFIDRTFLPIVAKL 123

Query: 133 AGQLTTVDKTLLLTDGPDKTAELPGLVGEYEQLLAAASPRYDFPDFDENSVATTFYTTGT 192
              L+TV   +L+    ++ + +   +  Y++++A+    Y +P  DE+  ++  YT+GT
Sbjct: 124 RSALSTVKHVILMGPRDEEASGMLDGLRFYDEVVASGDADYPWPSIDEDLPSSLCYTSGT 183

Query: 193 TGNPKGVYFSHRQLVLHTLAEASVTGSIDSVRLLGSNDVYMPITPMFHVHAWGIPYAATM 252
           TG+PKGV +SHR  VLH LA  S  G   S R     D  MP+ PMFHV+AWG+PY A  
Sbjct: 184 TGHPKGVLYSHRSTVLHALAGNSPDGIGVSAR-----DAVMPVVPMFHVNAWGVPYIAAA 238

Query: 253 LGMKQVYPG-RYEPDMLVKLWREEKVTFSHCVPTILQMLLNCPNAQGQDFGGWK-IIIGG 310
            G K V PG   +   L  L  +E+V  +  VPTI   LL    A+G      K  I+GG
Sbjct: 239 QGAKLVLPGPNLDGPSLATLIDDEEVGLALGVPTIWMGLLAALRARGTKPKSLKRTIVGG 298

Query: 311 SSLNRSLYQAALAR-GIQLTAAYGMSETCPLIS-----AAHLNDELQAGSEDERVTYRIK 364
           ++L  S++ A     G++L  A+GM+ET PL +     A H++ + Q  SE      R+ 
Sbjct: 299 AALPPSMFAAFRDEFGVELIHAWGMTETSPLGTLNQPLARHIHLDPQRQSE-----VRLG 353

Query: 365 AGVPVPLVEAAIVDGEGNFLPADGETQGELVLRAPWLTMGYFKEPEKSEELWQGGWLHTG 424
            G P   V+  +VD  G  LP DG++ G+L +R  W+   YF    +S  L   GW  TG
Sbjct: 354 QGRPPYGVQLRVVDDGGAVLPNDGKSPGKLQIRGHWIVSSYF--GAESSALTSDGWFDTG 411

Query: 425 DVATLDGMGYIDIRDRIKDVIKTGGEWVSSLDLEDLISRHPAVREVAVVGVADPQWGERP 484
           D+ATLD  GY+ IRDR KD+IK+GGEW+SS+D+E++   HPA+   A +GV  P+W ERP
Sbjct: 412 DIATLDANGYMVIRDRAKDIIKSGGEWISSVDIENIAIGHPAIANAAAIGVPHPKWDERP 471

Query: 485 FALLVARDGHDIDAKALKEHLKPFVEQGHINKWAIPSQIALVTEIPKTSVGKLDKKRIRQ 544
             + V ++G ++    L    K     G +  W IP +   +  +P +  GK+ K R+R+
Sbjct: 472 VLVAVKKEGANVSEADLLAFFK-----GRLPDWQIPDKAVFIDALPLSGTGKVFKNRLRE 526