Pairwise Alignments

Query, 560 a.a., Medium-chain-fatty-acid CoA ligase from Pseudomonas putida KT2440

Subject, 518 a.a., Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) from Variovorax sp. SCN45

 Score =  222 bits (566), Expect = 2e-62
 Identities = 158/533 (29%), Positives = 264/533 (49%), Gaps = 39/533 (7%)

Query: 19  LIKRLLMSGSRYEKTREIVYRDQMRLTYPQLNERIARLANVLTEAGVKAGDTVAVMDWDS 78
           L+KR +   ++   T   +  D+ R T+ ++ ER+ R+A  L   GV+ G  VA++  +S
Sbjct: 6   LLKRAVQCSAQRIAT---ISGDRQR-TWKEVGERVPRMAAALRSLGVRDGAFVAILAMNS 61

Query: 79  HRYLECMFAIPMIGAVVHTINVRLSPEQILYTMNHAEDRVVLVNSDFVGLYQAIAGQLTT 138
            RYLE  F++P  G  +  +N+R S  + +Y +  +   V+ V+  F+     +  +  +
Sbjct: 62  DRYLELFFSVPWAGGALAPLNIRWSAVENVYALTDSNAEVLFVDDSFLDQMDFLRAERPS 121

Query: 139 VDKTLLLTDGPDKTAELPGLVGEYEQLLAAASPRYDFPDFDENSVATTFYTTGTTGNPKG 198
           +   + + DGP     L      YE+L+A  +P  D  D  E+ +   FYT GTT +PKG
Sbjct: 122 IRHVIYMGDGPTPAGMLA-----YEELVAQHAPMPD-ADRKEDDLYVIFYTGGTTAHPKG 175

Query: 199 VYFSHRQLVLHTLAEASVTGSIDSVRLLGSNDVYMPITPMFH-VHAWGIPYAATMLGMKQ 257
           V  SHR + L T    ++  S++ +        ++ +   FH   A  + Y     G   
Sbjct: 176 VAMSHRGVYLATCCYLALLPSVEDL-------TFLYVAGYFHFAGASALWYITLAAGTHA 228

Query: 258 VYPGRYEPDMLVKLWREEKVTFSHCVPTILQMLLNCPNAQGQDFGGWKIIIGGSSLNRSL 317
           + P ++E   ++K   + +VT +  VPT++ MLL+ P+ +  D    K  I G S    +
Sbjct: 229 ILP-KFEALPVMKAIGQHRVTNAVLVPTMVNMLLSHPDFKQYDLTSLKTCIYGGS---PM 284

Query: 318 YQAALARGIQLTAA------YGMSETCPLISAAHLNDELQAGSEDERVTYRIKAGVPVPL 371
            +  + R +++  +      YGM+ET    +     D + +G    R+    +AG     
Sbjct: 285 PEGLMLRAMKMVPSWGFYQIYGMTETGGFATMLRWRDHIVSGENASRLRSAGQAGFGN-- 342

Query: 372 VEAAIVDGEGNFLPADGETQGELVLRAPWLTMGYFKEPEKSEELWQGGWLHTGDVATLDG 431
            E  +V  EG+   AD    GE+++R+ +L MGY   PE +    + GW+HTGD   LD 
Sbjct: 343 -EVRVVRIEGD--DADVGEIGEIIVRSDFLMMGYLNNPEATAACLRDGWMHTGDAGYLDA 399

Query: 432 MGYIDIRDRIKDVIKTGGEWVSSLDLEDLISRHPAVREVAVVGVADPQWGERPFALLVAR 491
            G++ + DR+KD+I +GGE V S++ E  +  HPAVRE AV+G+   QWGE   A++V +
Sbjct: 400 DGFLYVADRVKDMIVSGGENVYSIEPERALFLHPAVREAAVIGIPSEQWGESVHAVVVLK 459

Query: 492 DGHDIDAKALKEHLKPFVEQGHINKWAIPSQIALVTE-IPKTSVGKLDKKRIR 543
           DG    A+ L  H +       I  +  P  I    + +P T VGK+ K  +R
Sbjct: 460 DGASATAEELIAHCRTL-----IGGYKCPRSIEFRADPLPVTPVGKVRKNVLR 507