Pairwise Alignments

Query, 560 a.a., Medium-chain-fatty-acid CoA ligase from Pseudomonas putida KT2440

Subject, 546 a.a., 3-methylmercaptopropionyl-CoA ligase (EC 6.2.1.44) of DmdB2 type from Variovorax sp. SCN45

 Score =  356 bits (913), Expect = e-102
 Identities = 213/550 (38%), Positives = 316/550 (57%), Gaps = 25/550 (4%)

Query: 17  PLLIKRLLMSGSRYEKTREIVYRDQM----RLTYPQLNERIARLANVLTEAGVKAGDTVA 72
           PLLI  L+   + +    E+V R       R  Y ++  R  ++AN L   GV++GD V 
Sbjct: 9   PLLISSLIDYAATFHPHAEVVGRTVEGPVHRTNYSEIQRRSKQVANALRTLGVESGDRVG 68

Query: 73  VMDWDSHRYLECMFAIPMIGAVVHTINVRLSPEQILYTMNHAEDRVVLVNSDFVGLYQAI 132
            + W+++R+L   F +   GAV+HT+N RL PEQI Y +NHAED+V+  ++ F  L + +
Sbjct: 69  TLAWNTYRHLALYFGVSGSGAVLHTVNPRLFPEQIDYIVNHAEDKVLFFDTTFAPLVEKL 128

Query: 133 AGQLTTVDKTLLLTDGPDKTA-ELPGLVGEYEQLLAAASPRYDFPDFDENSVATTFYTTG 191
           A  L +V   + +TD     A ++P L+  Y++L+ A S  YD+P+FDE + ++  YT+G
Sbjct: 129 APGLKSVRAFIAMTDRAHMPAIDVPNLLC-YDELVGAQSEAYDWPEFDERTASSLCYTSG 187

Query: 192 TTGNPKGVYFSHRQLVLHTLAE-ASVTGSIDSVRLLGSNDVYMPITPMFHVHAWGIPYAA 250
           TTGNPKGV +SHR  VLH+L E A  T  ++S   L      +   PMFH +AWG+PYAA
Sbjct: 188 TTGNPKGVLYSHRSTVLHSLMECAPDTFGVNSQMTL------LLAVPMFHANAWGMPYAA 241

Query: 251 TMLGMKQVYPGRY-EPDMLVKLWREEKVTFSHCVPTILQMLLNC----PNAQGQDFGGWK 305
            M GMK V PG + +   L +L R+EKV FS  VPTI  ML       P    +  G  +
Sbjct: 242 AMTGMKLVMPGPHMDGQSLYELMRDEKVGFSQAVPTIWLMLFQYFDAHPEIDTRAIGLKR 301

Query: 306 IIIGGSSLNRSLYQAALAR-GIQLTAAYGMSETCPLISAAHLNDELQAGSEDERVTYRIK 364
           I +GG++  RS+ +      G      +GM+ET P+    +L  +  +  ++E++  +++
Sbjct: 302 IGVGGAATPRSMIERFERDFGADFVQGWGMTETSPIGVIGNLLPKHLSMPKEEQLRVKMR 361

Query: 365 AGVPVPLVEAAIVDGEGNFLPADGETQGELVLRAPWLTMGYFKEPEKSEELWQGGWLHTG 424
            G  V  VE  IV  +G  LP DG+  G L +R PW+  GYFK  E    L   G+  TG
Sbjct: 362 QGRGVWGVELKIVGEDGQRLPHDGKAFGHLHVRGPWIASGYFKS-EGGPVLDAEGFFPTG 420

Query: 425 DVATLDGMGYIDIRDRIKDVIKTGGEWVSSLDLEDLISRHPAVREVAVVGVADPQWGERP 484
           DVA +D  GY+ + DR KDVIK+GGEW+SS+DLE+  S HPAV E AV+G+A P+W ERP
Sbjct: 421 DVANIDEDGYVQLVDRAKDVIKSGGEWISSIDLENTASLHPAVAEAAVIGIAHPKWQERP 480

Query: 485 FALLVARDGHDIDAKALKEHLKPFVEQGHINKWAIPSQIALVTEIPKTSVGKLDKKRIRQ 544
             ++V R G ++     +E L  F+ +  + KW +P  +  V  +P T+ GKL K ++R+
Sbjct: 481 LLIVVKRAGSEV----TREELLTFLAE-RVVKWWLPEDVVFVDSLPHTATGKLLKTKLRE 535

Query: 545 DIVQWQASNS 554
               + A+ S
Sbjct: 536 QFKGYTATTS 545