Pairwise Alignments
Query, 1597 a.a., type IV secretion protein Rhs from Pseudomonas fluorescens SBW25
Subject, 1426 a.a., rhsD element protein (NCBI) from Escherichia coli BW25113
Score = 205 bits (521), Expect = 3e-56
Identities = 244/930 (26%), Positives = 368/930 (39%), Gaps = 148/930 (15%)
Query: 616 YAYDGAGRLVSATNAVGESEVYRYDEQHV----------------------ILERGLAGG 653
Y YD GR+V N G S Y Y++ + ++++ LA G
Sbjct: 363 YRYDDTGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADG 422
Query: 654 ASFYWEWEQAGKAARCVRHWASFSQMDTRYAWDDNGQVTVFNADGSQEIYVHDQRARLVQ 713
+ ++ AG+ ++ D +T DG + + ++ +L
Sbjct: 423 SVTRSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITT--PDGRETKFYYNDGNQLTA 480
Query: 714 RVDPDGAEHFKSYDEKGRLTVEQDPLGAVKAYQYDDA----------------------- 750
V PDG E + YDE GRL E G Y+YDDA
Sbjct: 481 VVSPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRY 540
Query: 751 ARLVALFPGEDEPTTYEHDN-GFVRVVRRGEAVWKYERND-QGDVIRRTDPDGNATDYSY 808
+L+A T YE+D G + V R E + Y R D +G + D G T Y Y
Sbjct: 541 GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEY 600
Query: 809 NKYGQLVGVWYPDHSCQTLVWNERGQLLEEQCPNGGIKR-YRYDDLGRKISQEDEHGALT 867
N G L V PD + ++ G+ + GG+ R YD GR IS +E+G+ +
Sbjct: 601 NAAGDLTAVITPDGNRSETQYDAWGKAVSTT--QGGLTRSMEYDAAGRVISLTNENGSHS 658
Query: 868 QFEWNDVGRLVQIVLPSGDTRKYTYNPYGKITSERDELGNITRYEYADGLHLISRRLNAD 927
F ++ + RLVQ G T++Y Y+ GK+T DE G + + Y + + R +N +
Sbjct: 659 VFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDE-GLVILWYYDESDRITHRTVNGE 717
Query: 928 GSQVNYRYDNVRLLLTEIENEVGETYRLQYHSNGLIQQETGFDGQRTAYVYDLNGNL--Q 985
++ ++YD LT+I + + E +R+ H + G+ G L Q
Sbjct: 718 PAE-QWQYDG-HGWLTDISH-LSEGHRVAVHYG--YDDKGRLTGECQTVENPETGELLWQ 772
Query: 986 EKTEHGDDGSQLVTRYERDH----------AGRLVRKTLPDGNSVNYAYDR-------QG 1028
+T+H + L R D +G L L V Y DR
Sbjct: 773 HETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSF 832
Query: 1029 NLLSVDDGNWFLAYEYDVQNRLTAEH---------QGWG-------------TLRYGYDA 1066
++ + + L Y +L ++H GW T YGY A
Sbjct: 833 GSMAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWSDNGDLVRISGPRQTREYGYSA 892
Query: 1067 CGQLTNLRL--PDNN-RLTFNHDKGGH-LSTVELN-GAVLTSHQFNADREQQRTQGKLLS 1121
G+L ++R PD + R+ + D G+ L EL+ + LT N E
Sbjct: 893 TGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHY------ 946
Query: 1122 TYQHDHQGRLFNQSIGDAEGPVYRRHYDYDKSGNLTRLLDTRKGEHHYHYDPLSRL---T 1178
Y+HD GRL ++ G + R D R HHYHYD RL T
Sbjct: 947 VYRHDEYGRLTEKTDRIPAGVI--------------RTDDERT--HHYHYDSQHRLVFYT 990
Query: 1179 RADHSQGEQE-RFGHDPAGNLLMQN--RPGPDIVAGNRLMIQGDHQYDYDAFGNLIRERR 1235
R H + E R+ +DP G + + R D+ L + + + Y G+ + +
Sbjct: 991 RIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTW-YGWDGDRLTTVQ 1049
Query: 1236 GKGHRLVTEYRYDCQHRLIGTKKPNGQTASYRYDPFGRRISKTVGD------VTTEFFWK 1289
R+ T Y LI + NG+ + + + + E
Sbjct: 1050 TDTTRIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGHGVVFPAELVRL 1109
Query: 1290 GDTLIAEHHAER----HRSYLYEPDSFRPLALLEGFGPKET---KAYHYQLDHLGTPQEL 1342
D L E A+R R++L + L P+ T KA+ Y DH G P L
Sbjct: 1110 LDRLEEEIRADRVSSESRAWLAQC-GLTVEQLARQVEPEYTPARKAHLYHCDHRGLPLAL 1168
Query: 1343 TAADGEIVWSAHYRAYG-EITRLDIGKIDNPLRFQGQYFDQESGLHYNRHRYYHPDIGRY 1401
+ DG WSA Y +G ++ + + P R GQ D+ESGL+YNRHRYY P GRY
Sbjct: 1169 ISEDGNTAWSAEYDEWGNQLNEENPHHVYQPYRLPGQQHDEESGLYYNRHRYYDPLQGRY 1228
Query: 1402 LTPDPVKLTGGINAYQYVPNPTGWVDPLGL 1431
+T DP+ L GG N YQY NP +DP+GL
Sbjct: 1229 ITQDPMGLKGGWNLYQYPLNPLQQIDPMGL 1258
Score = 62.0 bits (149), Expect = 5e-13
Identities = 93/390 (23%), Positives = 139/390 (35%), Gaps = 80/390 (20%)
Query: 1012 KTLPDGNSVNYAYDRQGNLLSVDD--GNWFLAYEYDVQN--RLTA-EHQGWGTLRYGYDA 1066
++LP V Y Y G LL+V D A+ YD Q+ R+ A + G +RY YD
Sbjct: 308 ESLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDD 367
Query: 1067 CGQLTNLRLP---------DNNRLTFN---------HDKGG----HLSTVELNGAVLTSH 1104
G++ P + +R+T H +GG + EL +T
Sbjct: 368 TGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRS 427
Query: 1105 QFNADREQQRTQGKLLSTYQHDHQGR-------LFNQSIGDAEGPVYRRHYDYDKSGN-L 1156
++A G+L T Q D GR + + I D P R Y GN L
Sbjct: 428 GYDA-------AGRL--TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQL 478
Query: 1157 TRLLDTRKGEHHYHYDPLSRLTRADHSQGEQERFGHDPAGNLLMQNRPGPDIVAGNRLMI 1216
T ++ E YD RL GE R+ +D A + L D R M
Sbjct: 479 TAVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAHSELPATT--TDATGSTRQMT 536
Query: 1217 QGDH--------------QYDYDAFGNLIRERRGKGHRLV-------------------T 1243
+ +Y+YD FG + R +G L T
Sbjct: 537 WSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRET 596
Query: 1244 EYRYDCQHRLIGTKKPNGQTASYRYDPFGRRISKTVGDVTTEFFWKGDTLIAEHHAERHR 1303
Y Y+ L P+G + +YD +G+ +S T G +T + + E
Sbjct: 597 RYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGS 656
Query: 1304 SYLYEPDSFRPLALLEGFGPKETKAYHYQL 1333
++ D+ L GF + T+ YHY L
Sbjct: 657 HSVFSYDALDRLVQQGGFDGR-TQRYHYDL 685
Score = 35.8 bits (81), Expect = 4e-05
Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Query: 416 PNGDAAAFSDKTATNGCPVSMVTGEELLT-LTDGALDGVLPFEWTRLYRTSAVEVDCGLG 474
P G A + T+G PV+ + G ++L TD AL G LPF +R Y + + +G
Sbjct: 31 PTGVACSVCPGGMTSGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVG 90
Query: 475 F---GWSHSLAHRLAVSGNSVVWTDHENRS 501
GW RL + + ++ D+ RS
Sbjct: 91 VFGPGWKAPSDIRLQLRDDGLILNDNGGRS 120