Pairwise Alignments
Query, 1597 a.a., type IV secretion protein Rhs from Pseudomonas fluorescens SBW25
Subject, 1411 a.a., Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor from Escherichia coli BL21
Score = 206 bits (524), Expect = 1e-56
Identities = 245/948 (25%), Positives = 370/948 (39%), Gaps = 175/948 (18%)
Query: 609 TEQTVVSYAYDGAGRLVSATNAVGESEVYRYDEQHVILERGLAGGASFYWEWEQAGKAAR 668
T + + Y YD GR+ N G S Y+Y++ H+ + L + + E K
Sbjct: 352 TGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVV 411
Query: 669 CVRHW---ASFSQMDT----RYAWDDNGQVTVFNAD-------------GSQEIYVHDQR 708
H + SQ D R D G+ T ++ D G + ++
Sbjct: 412 KKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHH 471
Query: 709 ARLVQRVDPDGAEHFKSYDEKGRLTVEQDPLGAVKAYQYDDA------------------ 750
+L PDG E + YDE GRL E P G + Y+YD+
Sbjct: 472 NQLTSATGPDGLELRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTM 531
Query: 751 -----ARLVALFPGEDEPTTYEHDN-GFVRVVRRGEAVWKYERND-QGDVIRRTDPDGNA 803
+L++ T Y+HD G + V R E + +Y D +G +I D G+
Sbjct: 532 TWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHE 591
Query: 804 TDYSYNKYGQLVGVWYPDHSCQTLVWNERGQLLEEQCPNGGIKR-YRYDDLGRKISQEDE 862
T Y YN G L V PD S ++ G+ + + GG+ R YD GR I E
Sbjct: 592 TRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAV--RTTQGGLTRSMEYDAAGRVIRLTSE 649
Query: 863 HGALTQFEWNDVGRLVQIVLPSGDTRKYTYNPYGKITSERDELGNITRYEYADGLHLISR 922
+G+ T F ++ + RL+Q G T++Y ++ GK+ DE G +T + Y + L R
Sbjct: 650 NGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDE-GLVTHWHYDEADRLTHR 708
Query: 923 RLNADGSQVNYRYDNVRLLLTEIENEVGETYRLQYHSNGLIQQETGFDGQRTAYVYDLNG 982
+ + ++ ++YD R LT+I + + E +R+ H Y YD G
Sbjct: 709 TVKGETAE-RWQYDE-RGWLTDISH-ISEGHRVAVH-----------------YRYDEKG 748
Query: 983 NL--QEKTEHGDDGSQLVTRYERDHA-------GRLVRKTLP------------------ 1015
L + +T H + L+ ++E HA R + +LP
Sbjct: 749 RLTGERQTVHHPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLG 808
Query: 1016 DGNSVNYAYDRQGNLLSVDDGNWFLAYEYDVQNRLTAEHQGWGTLRYGYDAC----GQLT 1071
D V Y DR G + L+ Y +L ++H +L+Y D G+L
Sbjct: 809 DTPLVEYTRDRLHRETLRSFGRYELSTAYTPAGQLQSQHLN--SLQYDRDYTWNDNGELI 866
Query: 1072 NLRLPDNNRLTFNHDKGGHLSTVELNGAVL-------TSHQFNADREQQRTQGKLLST-- 1122
+ P R ++++ G L++V A L T N + + LS
Sbjct: 867 RISSPRQTR-SYSYSTTGRLTSVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWP 925
Query: 1123 -----------YQHDHQGRLFNQSIGDAEGPVY----RRH-YDYDKSGNLTRLLDTRKGE 1166
Y++D GRL ++ EG + R H Y YD L T+ E
Sbjct: 926 DNRIARDAHYLYRYDRYGRLTEKTDLIPEGVIRTDDERTHQYHYDSQHRLVHYTRTQYAE 985
Query: 1167 ----HHYHYDPLSRLTRADHSQGEQERFGHDPAGNLLMQNRPGPDIVA--GNRLM-IQGD 1219
Y YDPL R R +ER D G + + +P G+RL IQ D
Sbjct: 986 PLVESRYLYDPLGR--RVAKRVWRRER---DLTGWMSLSRKPEVTWYGWDGDRLTTIQND 1040
Query: 1220 HQ-----YDYDAFGNLIRERRGKGHRLVTEYRYDCQHRLIGTKKPNG--QTASYRYDP-- 1270
Y +F LIR G T+ R L T + +G S + P
Sbjct: 1041 RTRIQTIYQPGSFTPLIRVETATGELAKTQRR-----SLADTLQQSGGEDGGSVVFPPVL 1095
Query: 1271 ---FGRRISKTVGDVTTEFF--WKGDTLIAEHHAERHRSYLYEPDSFRPLALLEGFGPKE 1325
R S+ + D +E W + + +Y P
Sbjct: 1096 VQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTP---------------A 1140
Query: 1326 TKAYHYQLDHLGTPQELTAADGEIVWSAHYRAYGEITRLDIG-KIDNPLRFQGQYFDQES 1384
K + Y DH G P L + +G W A Y +G + + ++ +R GQ +D+ES
Sbjct: 1141 RKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES 1200
Query: 1385 GLHYNRHRYYHPDIGRYLTPDPVKLTGGINAYQYVPNPTGWVDPLGLS 1432
GL+YNRHRYY P GRY+T DP+ L GG N Y Y NP +DPLGLS
Sbjct: 1201 GLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLS 1248
Score = 110 bits (275), Expect = 1e-27
Identities = 126/534 (23%), Positives = 209/534 (39%), Gaps = 98/534 (18%)
Query: 764 TTYEHDNGFVRVVRRGEAVWKYERNDQGDVIRRTDPDGNATDYSYNKYGQLVGVWYPDHS 823
T Y DNG R AVW + + + P Y + G+L V+ ++
Sbjct: 281 TEYGRDNGI-----RLSAVWLTHDPEYPENL----PAAPLVRYGWTPRGELAAVYDRSNT 331
Query: 824 -CQTLVWNE--RGQLLEEQCPNGGIKRYRYDDLGRKISQEDEHGALTQFEWNDVGRLVQI 880
++ +++ RG+++ + RYRYD GR Q
Sbjct: 332 QVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQ--------------------- 370
Query: 881 VLPSGDTRKYTYNPYGKITSERDELGNITRYEYADGLHLISRRLNADGSQVNYRYDNVRL 940
+ P+G + Y Y ++ + + + GL + ++ +ADGS ++D V
Sbjct: 371 LNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGR 430
Query: 941 LLTEIENEVGETYRLQYHSNGLIQQETGFDGQRTAYVYDLNGNLQEKTEHGDDGSQLVTR 1000
L T+ + T GLI + T DG+ +A+ Y+ + L T G DG +L R
Sbjct: 431 LRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT--GPDGLEL--R 486
Query: 1001 YERDHAGRLVRKTLPDGNSVNYAYD-----------------------RQGNLLSVDDGN 1037
E D GRL+++T PDG+ Y YD R G LLS D +
Sbjct: 487 REYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS 546
Query: 1038 WFLA-YEYDVQNRLTAEHQGWGTLRY-GYDACGQLTNLRLPDNNRLTFNHDKGGHLSTVE 1095
++ Y++D ++TA H+ G +Y YD+ GQL ++ + + ++ G L+ V
Sbjct: 547 GYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVI 606
Query: 1096 LNGAVLTSHQFNA-DREQQRTQGKLLSTYQHDHQGRLFNQSIGDAEGPVYRRHYDYDKSG 1154
Q++A + + TQG L R +YD +G
Sbjct: 607 APDGSRNGTQYDAWGKAVRTTQGGL-------------------------TRSMEYDAAG 641
Query: 1155 NLTRLLDTRKGEHHYHYDPLSRLTRADHSQGEQERFGHDPAGNLLMQNRPG--------- 1205
+ RL + YD L RL + G +R+ HD G L+ G
Sbjct: 642 RVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDE 701
Query: 1206 PDIVAGNRLMIQGDHQYDYDAFGNLIR-ERRGKGHRLVTEYRYDCQHRLIGTKK 1258
D + + + ++ YD G L +GHR+ YRYD + RL G ++
Sbjct: 702 ADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQ 755
Score = 65.1 bits (157), Expect = 5e-14
Identities = 113/543 (20%), Positives = 191/543 (35%), Gaps = 118/543 (21%)
Query: 999 TRYERDHAGRLVR----------KTLPDGNSVNYAYDRQGNLLSVDD--GNWFLAYEYDV 1046
T Y RD+ RL + LP V Y + +G L +V D ++ YD
Sbjct: 281 TEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDD 340
Query: 1047 QNR---LTAEHQGWGTLRYGYDACGQLTNLRLPDNNRLTFNHDKGGHLSTVELNGAVLTS 1103
+ R + H G +RY YD+ G++T P T+ ++K T L+ +
Sbjct: 341 KYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLH 400
Query: 1104 HQFNADR----EQQRTQGKLLSTYQHDHQGRLFNQSIGDAEGPVYRRHYDYDKSGNLTRL 1159
Q A +++ G + + Q D GRL Q+ DA G D +G +TR+
Sbjct: 401 TQGEAGLKRVVKKEHADGSVTQS-QFDAVGRLRTQT--DAAGRTTEYSPDV-VTGLITRI 456
Query: 1160 LDTRKGEHHYHYDPLSRLTRADHSQGEQERFGHDPAGNLLMQNRPGPDI----------- 1208
++Y+ ++LT A G + R +D G L+ + P DI
Sbjct: 457 TTPDGRASAFYYNHHNQLTSATGPDGLELRREYDELGRLIQETAPDGDITRYRYDNPHSD 516
Query: 1209 -------VAGNRLMIQGDH---------------QYDYDAFGNLIRERRGKGHRLVTEYR 1246
G+R + +YD+D FG + R +G +++YR
Sbjct: 517 LPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEG---LSQYR 573
Query: 1247 -YDCQHRLIGTKKPNGQTASY---------------------RYDPFGRRISKTVGDVTT 1284
YD + +LI K G Y +YD +G+ + T G +T
Sbjct: 574 AYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTR 633
Query: 1285 EFFWKGDTLIAEHHAERHRSYLYEPDSFRPLALLEGFGPKETKAYHYQLDHLGTPQELTA 1344
+ + +E + D L GF + T+ YH+ L T + + +
Sbjct: 634 SMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGR-TQRYHHDL----TGKLIRS 688
Query: 1345 ADGEIVWSAHYRAYGEITR-------------------LDIGKIDNPLRFQGQY-FDQES 1384
D +V HY +T DI I R Y +D++
Sbjct: 689 EDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHYRYDEKG 748
Query: 1385 GLHYNRHRYYHPDIGRYLTPDPVK------------LTGGINAYQYVPNPTGWVDPLGLS 1432
L R +HP+ L + + + A +++ +GW+ + L
Sbjct: 749 RLTGERQTVHHPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLG 808
Query: 1433 SCP 1435
P
Sbjct: 809 DTP 811
Score = 38.5 bits (88), Expect = 5e-06
Identities = 30/90 (33%), Positives = 43/90 (47%), Gaps = 4/90 (4%)
Query: 416 PNGDAAAFSDKTATNGCPVSMVTGEELLT-LTDGALDGVLPFEWTRLY---RTSAVEVDC 471
P G A + T+G PV+ + G ++L TD AL LPF +R Y RT
Sbjct: 31 PTGVACSVCPGGVTSGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVG 90
Query: 472 GLGFGWSHSLAHRLAVSGNSVVWTDHENRS 501
LG GW RL + N+++ +D+ RS
Sbjct: 91 SLGPGWKMPADIRLQLRDNTLILSDNGGRS 120