Pairwise Alignments

Query, 677 a.a., Methionine--tRNA ligase from Escherichia coli HS(pFamp)R (ATCC 700891)

Subject, 679 a.a., Methionine--tRNA ligase from Pseudomonas putida KT2440

 Score =  920 bits (2379), Expect = 0.0
 Identities = 446/676 (65%), Positives = 546/676 (80%), Gaps = 5/676 (0%)

Query: 6   KKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGHEVNFICADDAHGTPIMLKAQQ 65
           ++ILVT ALPYANGSIHLGHMLE+IQ D+WVR+Q++RG++  ++CADDAHG+ IML+A++
Sbjct: 5   RQILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQCIYVCADDAHGSAIMLRAEK 64

Query: 66  LGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEENRQLSELIYSRLKENGFIKNRTI 125
            GITPEQ+I  +  EH +DFA F + +DN+HSTHS+ENR+LS LIYSRL+E G I  R++
Sbjct: 65  EGITPEQLIANVQAEHSSDFADFLVDFDNFHSTHSDENRELSSLIYSRLREAGHIATRSV 124

Query: 126 SQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIEPKSVVSGATPVM 185
           +Q +DPEKGMFL DRF+KGTCPKC + DQYGDNCE CGATY+PTEL  PKS +SGATPV+
Sbjct: 125 TQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTELKNPKSAISGATPVL 184

Query: 186 RDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDAPYFGFEIPN 245
           RDS+HFFF LP F  MLQ WTRSG LQ+ VANK+ EW +SGLQ+WDISRDAPYFGFEIP 
Sbjct: 185 RDSQHFFFKLPDFQAMLQQWTRSGTLQDAVANKLAEWLDSGLQEWDISRDAPYFGFEIPG 244

Query: 246 APGKYFYVWLDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYHFIGKDIVYFHSLF 305
            PGKYFYVWLDAPIGYM SFKNLC +R + + FD +W + S AELYHFIGKDIV FH+LF
Sbjct: 245 EPGKYFYVWLDAPIGYMASFKNLCARRPE-LDFDAFWHEGSKAELYHFIGKDIVNFHALF 303

Query: 306 WPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFDADSLRYYYTAKLS 365
           WPAMLEG+ FRKPT + VHGY+TVNGAKMSKSRGTFIKA T+L+H   + LRYYY AKL 
Sbjct: 304 WPAMLEGAGFRKPTAVNVHGYLTVNGAKMSKSRGTFIKARTYLDHLQPEYLRYYYAAKLG 363

Query: 366 SRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGVLANELADPQLYKTFTDA 425
             +DD+DLNLEDFVQ+VN+D+V KVVN+ASR AGFI+K  +GV+    A P+L + F  A
Sbjct: 364 RGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNEGVMVGGDAAPELTEAFLAA 423

Query: 426 AEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGINLF 485
           A  I EA+E+R+FG+A+REIMALAD AN ++ ++APW +AKQEG+  ++QAIC+ GINLF
Sbjct: 424 APSIAEAYEARDFGRAMREIMALADRANAWIADKAPWSLAKQEGKQDEVQAICAQGINLF 483

Query: 486 RVLMTYLKPVLPKLTERAEAFLN-TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALV 544
           R L+ +LKPVLP L   AEAFLN   LTW+     L  H++NPFKAL +RI+  +VEA+V
Sbjct: 484 RQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHLTRLENHQLNPFKALMSRIEPAKVEAMV 543

Query: 545 EASKEEVKAAAAPV---TGPLADDPIQETITFDDFAKVDLRVALIENAEFVEGSDKLLRL 601
            ASKE++ AA A      G LA DP+   I FD FA VDLRVALI  AE V G+DKLL+L
Sbjct: 544 AASKEDLLAAEAKAPAGNGELAKDPLSAEIEFDTFAAVDLRVALIVKAEAVAGADKLLQL 603

Query: 602 TLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDI 661
           TLD+G E+RNVFSGI+SAYPDP  L GR T+MVANL PRKMRFG+SEGMVMAAGPGG++I
Sbjct: 604 TLDIGDERRNVFSGIKSAYPDPSKLEGRLTMMVANLKPRKMRFGVSEGMVMAAGPGGEEI 663

Query: 662 FLLSPDAGAKPGHQVK 677
           +LLSPD+GAKPG ++K
Sbjct: 664 YLLSPDSGAKPGQRIK 679